This European Standard specifies a method for the separation of fatty acid methyl esters (FAME) from middle distillates by liquid chromatography (LC) and for quantitative determination of the individual esters by gas chromatography (GC). This method is applicable to FAME of vegetable or animal origin that contain methyl esters between C14 to C24. These FAME are mainly composed of C16 - C18 fatty acids. This method is applicable whatever the origin of the middle distillate fuel. This test method has been evaluated for the separation and characterisation of FAME present at up to 5 % (V/V) in middle distillate fuel.