This European Standard describes the necessary steps to be performed before carrying outecotoxicity tests on wastes. The purpose of this European Standard is to provide guidance on thetaking of the sample, transport, storage of wastes and to define preparation, for the determination ofecotoxicological properties of wastes under the conditions specified in this European Standard bybiological testing either as raw wastes or water extracts from wastes. Sample preparation for otherapplications (e.g. assessment of waste effects on aquatic and terrestrial organisms in a disposalscenario) is not considered. Specifying a test battery to characterize ecotoxicologica