This International Standard describes a method for determining the effects of substances or contaminated soils on reproduction and on survival of the worm Enchytraeus albidus (Enchytraeidae). The animals are exposed to the substances by dermal and alimentary uptake using a defined artificial soil substrate to which specified amounts of that substance are added, or by using a soil substrate of unknown quality.This International Standard is applicable to test substances that are either insoluble or soluble in water, although the method of application differs.