This part of ISO 21427 specifies a method for assessing genotoxicity using Amphibia larvae (Xenopus laevis and Pleurodeles waltl). The provisions specific to Pleurodeles are described in Annex A.The method is applicable to:. aqueous effluents;. aqueous leachates;. eluates of soils;. eluates of industrial waste;. eluates of sludges from sewage treatment;. surface and ground water;. water-soluble substances.