DIN EN ISO 20418-1-2018

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 1: Peptide detection using LC-ESI-MS with protein reduction (ISO 20418-1:2018); German version EN ISO 20418-1:2018


DIN EN ISO 20418-1-2018 发布历史

This document specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC). This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e. g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated. This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series. This document does not apply if fibres of the same animal species are present (e. g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e. g. ISO 17751 series).

DIN EN ISO 20418-1-2018由德国标准化学会 DE-DIN 发布于 2018-0801。

DIN EN ISO 20418-1-2018在国际标准分类中归属于: 59.060.01 纺织纤维综合。

DIN EN ISO 20418-1-2018 发布之时,引用了标准

  • DIN EN ISO 1833-1-2011 纺织品.定量化学分析.第1部分:试验的一般原则(ISO 1833-1-2006,包括Cor 1-2009);德文版本EN ISO 1833-1-2010*2011-01-01 更新
  • DIN EN ISO 1833-2-2011 纺织品.定量化学分析.第2部分:三组分纤维混合物(ISO 1833-2-2006);德文版本EN ISO 1833-2-2010*2011-01-01 更新
  • DIN EN ISO 1833-3-2011 纺织品.定量化学分析.第3部分:纤维素和聚酯纤维的混合(硫酸法)(ISO 1833-3-2006);德文版本EN ISO 1833-3-2010*2011-01-01 更新
  • DIN EN ISO 1833-5-2011 纺织品.定量化学分析.第5部分:粘胶纤维,铜氨纤维或莫代尔纤维和棉纤维的混合物(锌酸钠法)(ISO 1833-5-2006);德文版本EN ISO 1833-5-2010*2011-01-01 更新
  • DIN EN ISO 1833-6-2011 纺织品.定量化学分析.第6部分:纤维粘胶或cupro,modal,lyocell纤维的某种类型和棉纤维的混纺物(蚁酸和氯化锌法)(ISO 1833-6-2007);德文版本EN ISO 1833-6-2010*2011-01-01 更新
  • DIN EN ISO 1833-8-2011 纺织品.定量化学分析.第8部分:醋酸纤维及三醋酸基纤维混织物(丙酮法)(ISO 1833-8-2006);德文版本EN ISO 1833-8-2010*2011-01-01 更新
  • DIN EN ISO 1833-9-2011 纺织品.定量化学分析.第9部分:醋酯与三醋酯纤维混纺产品的含量分析(苯甲醇法) (ISO 1833-9-2006);德文版本EN ISO 1833-9-2010*2011-01-01 更新
  • DIN EN ISO 1833-10-2011 纺织品.定量化学分析.第10部分:三醋酸或聚乳酸和某些其他纤维的混合物(二氯甲法)(ISO 1833-10-2006);德文版本EN ISO 1833-10-2010*2011-01-01 更新
  • DIN EN ISO 1833-12-2011 纺织品.定量化学分析.第12部分:丙烯酸纤维素,人造纤维,含氯纤维,弹性纤维及其他纤维混织物(二甲基甲酰胺法)(ISO 1833-12-2006);德文版本EN ISO 1833-12-2010*2011-01-01 更新
  • DIN EN ISO 1833-13-2011 纺织品.定量化学分析.第13部分:特定含氯纤维和某些其他纤维的混合物(二硫化碳/丙酮法)(ISO 1833-13-2006);德文版本EN ISO 1833-13-2010*2011-01-01 更新
  • DIN EN ISO 1833-14-2011 纺织品.定量化学分析.第14部分:乙酸酯和某些含氯纤维的混合物(醋酸法)(ISO 1833-14-2006);德文版本EN ISO 1833-14-2010*2011-01-01 更新
  • DIN EN ISO 1833-15-2011 纺织品.定量化学分析.第15部分:黄麻与某些动物纤维的混纺物(氮含量测定法)(ISO 1833-15-2006);德文版本EN ISO 1833-15-2010*2011-01-01 更新
  • DIN EN ISO 1833-16-2011 纺织品.定量化学分析.第16部分:聚丙烯纤维和某些其它纤维的混合(二甲苯法)(ISO 1833-16-2006);德文版本EN ISO 1833-16-2010*2011-01-01 更新
  • DIN EN ISO 1833-17-2011 纺织品.定量化学分析.第17部分:含氯纤维(聚氯乙烯)和其它纤维的混纺物(硫酸法)(ISO 1833-17-2006);德文版本EN ISO 1833-17-2010*2011-01-01 更新
  • DIN EN ISO 1833-18-2011 纺织品.定量化学分析.第18部分:丝绸和羊毛或头发的混合物(硫酸法)(ISO 1833-18-2006);德文版本EN ISO 1833-18-2010*2011-01-01 更新
  • DIN EN ISO 1833-19-2011 纺织品.定量化学分析.第19部分:纤维素纤维与石棉的混合物(加热法)(ISO 1833-19-2006);德文版本EN ISO 1833-19-2010*2011-01-01 更新
  • DIN EN ISO 1833-20-2011 纺织品.定量化学分析.第20部分:弹性纤维和某些其它纤维的混纺物(二甲基乙酰胺法)(ISO 1833-20-2009);德文版本EN ISO 1833-20-2010*2011-01-01 更新

* 在 DIN EN ISO 20418-1-2018 发布之后有更新,请注意新发布标准的变化。

DIN EN ISO 20418-1-2018的历代版本如下:

 

 

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标准号
DIN EN ISO 20418-1-2018
发布日期
1970年01月
实施日期
废止日期
国际标准分类号
59.060.01
发布单位
DE-DIN
引用标准
DIN EN ISO 137-2016 DIN EN ISO 1833-1-2011 DIN EN ISO 1833-2-2011 DIN EN ISO 1833-3-2011 DIN EN ISO 1833-4-2017 DIN EN ISO 1833-5-2011 DIN EN ISO 1833-6-2011 DIN EN ISO 1833-7-2017 DIN EN ISO 1833-8-2011 DIN EN ISO 1833-9-2011 DIN EN ISO 1833-10-2011 DIN EN ISO 1833-11-2017 DIN EN ISO 1833-12-2011 DIN EN ISO 1833-13-2011 DIN EN ISO 1833-14-2011 DIN EN ISO 1833-15-2011 DIN EN ISO 1833-16-2011 DIN EN ISO 1833-17-2011 DIN EN ISO 1833-18-2011 DIN EN ISO 1833-19-2011 DIN EN ISO 1833-20-2011 DIN EN IS
被代替标准
DIN EN ISO 20418-1-2017
适用范围
This document specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC). This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e. g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated. This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series. This document does not apply if fibres of the same animal species are present (e. g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e. g. ISO 17751 series).

DIN EN ISO 20418-1-2018系列标准





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