07.100.01 微生物学综合 标准查询与下载

ASTM E2186-02a(2010) 用彗星分析法测定真核细胞中DNA单线损伤的标准指南

A common result of cellular stress is an increase in DNA damage. DNA damage may be manifest in the form of base alterations, adduct formation, strand breaks, and cross linkages (19). Strand breaks may be introduced in many ways, directly by genotoxic compounds, through the induction of apoptosis or necrosis, secondarily through the interaction with oxygen radicals or other reactive intermediates, or as a consequence of excision repair enzymes (20-22). In addition to a linkage with cancer, studies have demonstrated that increases in cellular DNA damage precede or correspond with reduced growth, abnormal development, and reduced survival of adults, embryos, and larvae (16, 23, 24). The Comet assay can be easily utilized for collecting data on DNA strand breakage (9, 25, 26). It is a simple, rapid, and sensitive method that allows the comparison of DNA strand damage in different cell populations. As presented in this guide, the assay facilitates the detection of DNA single strand breaks and alkaline labile sites in individual cells, and can determine their abundance relative to control or reference cells (9, 16, 26). The assay offers a number of advantages; damage to the DNA in individual cells is measured, only extremely small numbers of cells need to be sampled to perform the assay (<10 000), the assay can be performed on practically any eukaryotic cell type, and it has been shown in comparative studies to be a very sensitive method for detecting DNA damage (2, 27). These are general guidelines. There are numerous procedural variants of this assay. The variation used is dependent upon the type of cells being examined, the types of DNA damage of interest, and the imaging and analysis capabilities of the lab conducting the assay. To visualize the DNA, it is stained with a fluorescent dye, or for light microscope analysis the DNA can be silver stained (28). Only fluorescent staining methods will be described in this guide. The microscopic determination of DNA migration can be made either by eye using an ocular micrometer or with the use of image analysis software. Scoring by eye can be performed using a calibrated ocular micrometer or by categorizing cells into four to five classes based on the extent of migration (29, 30). Image analysis systems are comprised of a CCD camera attached to a fluorescent microscope and software and hardware designed specifically to capture and analyze images of fluorescently stained nuclei. Using such a system, it is possible to measure the fluorescence intensity and distribution of DNA in and away from the nucleus (8). Using different procedural variants, the assay can be utilized to measure specific types of DNA alterations and DNA repair activity (1, 3, 8, 10, 13, 14, 17, 18). Alkaline lysis and electrophoresis conditions are used for the detection of single-stranded DNA damage, whereas neutral pH conditions facilitate the detection of double-strand breaks (31). Various sample treatments can be used to express specific types of DNA damage, or as in one method, to preserve strand damage at sites of DNA repair (10). Nuclease digestion steps can be used to introduce strand breaks at specific lesion sites. Using this approach, oxidative base damage can be detected by the use of endonuclease III (18), as well as DNA modifications resulting from exposure to ultraviolet light (UV) through the use of T4 endonuclease V (3). Modifications of this type vastly expand the utility of this assay and are good examples of its versatility. A sufficient knowledge of the biology of cel.......

Standard Guide for Determining DNA Single-Strand Damage in Eukaryotic Cells Using the Comet Assay

ASTM E2200-02 多孔生物控制有机体封装的信息的标准规范

1.1 This specification covers package information or information included with packages containing beneficial insects, beneficial mites or beneficial nematodes for biological control of pests, or a combination thereof.

Standard Specification for Information Included with Packaging of Multi-Cellular Biological Control Organisms

ANSI/AAMI ST 72-2002 细菌内毒素.试验方法学、日常检测和交替性批量测试

Specifies general criteria to be applied in the determination of bacterial endotoxins (pyrogens) on sterilized or sterilizable healthcare products, components or raw materials. Endotoxin methodologies covered include both qualitative (limit) methods and quantitative (end-point) methods. Determination of pyrogens other than bacterial endotoxins is not covered and acceptable levels for bacterial endotoxins are not covered.This standard was listed for public review in the 4/20/2001 issue of Standards Action. It is being resubmitted due to substantive changes to the text.

Bacterial endotoxins - Test methodologies,routine monitoring,and alternatives to batch testing

ASTM E2186-02a(2016) 使用彗星试验测定真核细胞中DNA单链损伤的标准指南

5.1 A common result of cellular stress is an increase in DNA damage. DNA damage may be manifest in the form of base alterations, adduct formation, strand breaks, and cross linkages (19). Strand breaks may be introduced in many ways, directly by genotoxic compounds, through the induction of apoptosis or necrosis, secondarily through the interaction with oxygen radicals or other reactive intermediates, or as a consequence of excision repair enzymes (20-22). In addition to a linkage with cancer, studies have demonstrated that increases in cellular DNA damage precede or correspond with reduced growth, abnormal development, and reduced survival of adults, embryos, and larvae (16, 23, 24). 5.1.1 The Comet assay can be easily utilized for collecting data on DNA strand breakage (9, 25, 26). It is a simple, rapid, and sensitive method that allows the comparison of DNA strand damage in different cell populations. As presented in this guide, the assay facilitates the detection of DNA single strand breaks and alkaline labile sites in individual cells, and can determine their abundance relative to control or reference cells (9, 16, 26). The assay offers a number of advantages; damage to the DNA in individual cells is measured, only extremely small numbers of cells need to be sampled to perform the assay (<108201;000), the assay can be performed on practically any eukaryotic cell type, and it has been shown in comparative studies to be a very sensitive method for detecting DNA damage (2, 27) . 5.1.2 These are general guidelines. There are numerous procedural variants of this assay. The variation used is dependent upon the type of cells being examined, the types of DNA damage of interest, and the imaging and analysis capabilities of the lab conducting the assay. To visualize the DNA, it is stained with a fluorescent dye, or for light microscope analysis the DNA can be silver stained (28). Only fluorescent staining methods will be described in this guide. The microscopic determination of DNA migration can be made either by eye using an ocular micrometer or with the use of image analysis software. Scoring by eye can be performed using a calibrated ocular micrometer or by categorizing cells into four to five classes based on the extent of migration (29, 30) . Image analysis systems are comprised of a CCD camera attached to a fluorescent microscope and software and hardware designed specifically to capture and analyze images of fluorescently stained nuclei. Using suc......

Standard Guide for Determining DNA Single-Strand Damage in Eukaryotic Cells Using the Comet Assay

DS/CEN/CR 14252:2001 微生物标准的协调 共同感兴趣的工作项目登记册

This report is designed to give a view of CEN Standards activity containing microbiological aspects. It takes the form of a register of work items. The register includes current work items and published standards from TCs which are working on standards containing microbiological aspects. The information in this register was produced on February 2001.

Co-ordination on microbiological Standards - Register of work items of common interest

ASTM E1493-92(1998) 噬菌体M13或其DNA鉴定的标准指南

Standard Guide for Identification of Bacteriophage M13 or Its DNA

ASTM E1531-00(2006) 用琼脂生长法直接检测细胞培养中枝原体属染菌的标准实施规程

1.1 This practice covers the procedures used for detection of mycoplasma contamination by direct microbiological culture.1.2 This practice does not cover indirect methods for detection of mycoplasma such as DNA staining, biochemical detection, or genetic probes.1.3 This practice does not cover methods for identification of mycoplasma organisms.1.4 This practice will not detect cultivar strains (1) of Mycoplasma hyorhinis.1.5 This practice is not intended for use in detection of mycoplasma contamination in sera, culture media, vaccines, or other systems.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium

STAS SR 13423-1999 用于食品行业的酶制剂过氧化物酶．酶的活性测定

Prezentul standard stabile?te metoda de analiz? pentru determinarea activit??ii enzimatice a peroxidazei, en-zim? implicat? ?n diferite procese biochimice din industria alimentar?: pentru testarea procesului de pasteuri-zare a laptelui, ca indicator de ca

Enzyme preparations for food industry -Peroxidase - Enzyme activity determination

STAS SR 13424-1999 用于食品行业的酶制剂．木瓜蛋白酶．酶的活性测定

Prezentul standard stabile?te metoda de analiz? pentru determinarea activit??ii enzimatice a papainei, enzim? utilizat? pentru prevenirea form?rii sedimentului sau a tulburelii pe parcursul depozit?rii berii, la producerea hidrolizatelor de cazein?, la am

Enzyme preparations for food industry Papain - Enzyme activity determination

GOST R 51426-1999 微生物学.饲料,混合饲料,饲料原料.微生物检验用稀释液制备的一般导则

Настоящий стандарт распространяется на корма, комбикорма, комбикормовое сырье и представляет собой общее руководство по приготовлению р

Microbiology. Feedstuffs, compound feeds, feed raw materials. General guidance for the preparation of dilutions for microbiological examination

JIS K 3836:1995 空气微生物取样器取样效率的检验方法

この規格は，バイオプロセスにおける空中浮遊菌の濃度測定に用いられる空中浮遊菌測定器の捕集性能を試験する方法について規定する。

Testing methods for collection efficiency of airborne microbe samplers

NF V08-027:1995 微生物学.预测病原耶尔森氏菌属及结肠耶氏菌素的一般原则

Microbiology. General guidance for the detection of presumptive pathogenic {Yersinia} {enterocolitica}.

ISO 10273:1994 微生物学 伪结肠耶尔森氏结肠杆菌病源检测的总则

Microbiology - General guidance for the detection of presumptive pathogenic Yersinia enterocolitica

JIS K 3602:1990 用微生物传感器评估生物化学需氧量(BODs)的仪器

この規格は，固定化微生物膜と酸素電極とを組み合わせた微生物電極を利用して発酵廃液，下水などの排水中の生物化学的酸素消費量(以下，BODsという。)値を測定するための計測器(以下，計測器という。)について規定する。

Apparatus for the estimation of biochemical oxygen demand (BODs) with microbial sensor

JIS K 3604:1990 组织培养用培养基(最小必需培养基)

この規格は，バィオテクノロジー関連分野において，動物組織・細胞を培養するときに用いる粉末培地のうち，イーグルの開発した最小必須培地(以下，粉末培地という。)の系統について規定する

Medium for tissue culture (minimum essential medium)

JIS K 3603:1990 冻藏和超低温保存用塑料瓶

この規格は，バイオテクノロジー関連分野において，細胞をその性質を変化させることなく， -80℃以下で凍結保存するために用いるプラスチック製 () 容器(以下，容器という。)について規定する。注 () ポリエチレン，ポリプロピレンなど。

Plastic vials for frozen storage and ultra low-temperature preservation

ASTM E1202-87(2008) 微核检测标准制定用的标准指南

Micronucleus assays for genetic damage have been developed in many types of eucaryotic cells, both in vitro and in vivo. The occurrence of micronuclei is indicative of chromosomal damage or mitotic spindle dysfunction.1.1 This guide covers minimal criteria which should be met by a micronucleus assay system prior to the development of an ASTM Standard or Guide for the conduct of that assay. 1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Guide for Development of Micronucleus Assay Standards

ASTM F838-83(1993) 测定液体过滤用膜过滤器的细菌保留率的标准试验方法

1.1 This test method determines the bacterial retention characteristics of membrane filters for liquid filtration using Pseudomonas diminuta as the challenge organism. This test may be employed to evaluate any membrane filter system used for liquid sterilization. 1.2 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Test Method for Determining Bacterial Retention Of Membrane Filters Utilized For Liquid Filtration

SANS 215-:2019 食品和动物饲料的微生物学．肠杆菌科检测和计数的水平法．第1部分：通过预浓缩MPN技术进行计数和检测

Specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. Enumerat

Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae Part 1: Detection and enumeration by MPN technique with pre-enrichment