13.060.70 水的生物学特性的检验 标准查询与下载

GSO ISO 11348-3:2013 水质水样对十字花科弧菌发光抑制效果的测定（发光细菌试验） 第3部分：使用冻干细菌的方法

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using freeze-dried bacteria. This method is applicable to: — waste water; — aqueous extracts and leachates; — fresh water (surface and ground water); — sea and brackish water; — eluates of sediment (freshwater, brackish and sea water); — pore water; — single substances, diluted in water.

Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 3: Method using freeze-dried bacteria

GSO ISO 10708:2013 水质 水介质中有机化合物最终需氧生物降解的评估 两个闭瓶测试阶段生化需氧量的估算

This International Standard specifies a method for the evaluation of the ultimate biodegradability by aerobic microorganisms of organic compounds, in particular poorly water-soluble compounds, at a given concentration. NOTE 1 The conditions described in this international Standard do not necessarily always correspond to the optimal conditions allowing the maximum degree of biodegradation to occur. The method applies to organic compounds which - are water soluble or poorly water soluble at the concentration used under the test conditions; - do not adsorb onto or have any effect on the oxygen electrode (see 8.1.2 and 8.3.4); - are not inhibitory to the test microorganisms at the concentration chosen for the test. NOTE 2 For special measures to introduce poorly water-soluble test compounds into the test vessels, see IS0 10634. NOTE 3 Inhibitory effects can be determined as described in 8.1.4 and 8.3.1, or by using any other method for determining the inhibitory effect on bacteria of a substance (e.g. IS0 8192).

Water quality -- Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds -- Determination of biochemical oxygen demand in a two-phase closed bottle test

GSO ISO/TS 20281:2013 水质 生态毒理学数据统计解释指南

This Technical Specification offers guidance on statistical methods used for the analysis of data of standardized ecotoxicity tests. It focuses on statistical methods for obtaining statistical estimates of parameters in current and future use, e.g. ECx (LCx), NOEC, NEC. The methods described are intended to cover laboratory ecotoxicity tests (aquatic, sediment and/or terrestrial tests), and may also be relevant for other toxicity tests. The main objective of this Technical Specification is to provide practical guidance on how to analyse the observations from ecotoxicity tests. Hypothesis testing, concentration-response modelling and biology-based modelling are discussed for the different data types (quantal, continuous and discrete data, corresponding to mortality, growth or reproduction). In addition, some guidance on experimental design is given. Although the main focus is on giving assistance to the experimentalist, a secondary aim is to help those who are responsible for evaluating toxicity tests. Finally, the document may be helpful in developing new toxicity test guidelines by giving information on experimental design and statistical analysis issues.

Water quality -- Guidance on statistical interpretation of ecotoxicity data

GSO ISO 9888:2013 水质 水介质中有机化合物最大需氧生物降解的评估 静态测试（Zahn-Velence 方法）

This International Standard specifies a method for the evaluation in aqueous medium of the ultimate biodegradability and, as additional information, the primary biodegradability and the total elimination from water, of organic compounds at a given concentration by aerobic microorganisms. The conditions described in this International Standard normally correspond to optimal conditions for allowing the maximum value of biodegradation to occur with the chosen inoculum in the test time. These conditions may even be more favourable than in full-scale wastewater treatment plants, especially if their hydraulic retention time, sludge age or the adaptation of the activated sludge is not optimal. The method applies to organic compounds which are a) water-soluble at the concentration used under the test conditions and not expected to be transformed to insoluble metabolites if biodegradation and not elimination only shall be determined; b) nonvolatile, or which have a negligible vapour pressure under the test conditions; c) not lost by foaming from the test solution; d) not inhibitory to the test microorganisms at the concentration chosen for the test. Inhibitory effects can be determined using a suitable test method (e.g. see ISO 8192). If the test compound is toxic, the test concentration must be lowered, or a pre-exposed inoculum can be used. This International Standard is also applicable to the measurement of biodegradation and elimination of dissolved organic compounds in wastewater (also called "test compound" in the method). NOTE If more information is required to predict the behaviour of test compounds or wastewater in a treatment plant, a simulation test (e.g. the activated sludge simulation test ISO 11733) should be performed. For appropriate use of this method and for alternative biodegradation methods, see ISO 15462.

Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)

GSO ISO 10872:2013 水质 评估沉积物和土壤样品对线虫的肥力、生长和繁殖的毒理学影响

This International Standard specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole fresh-water sediment (maximum salinity 5 ‰), soil and waste, as well as to pore water, elutriates and aqueous extracts that were obtained from contaminated sediment, soil and waste.

Water quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda)

GSO ISO 11348-1:2013 水质水样对甲壳类弧菌发光抑制效果的测定（发光细菌试验） 第1部分：新鲜菌的使用方法

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using freshly prepared bacteria. This method is applicable to: — waste water; — aqueous extracts and leachates; — fresh water (surface and ground water); — sea and brackish water; — eluates of sediment (fresh water, brackish and sea water); — pore water; — single substances, diluted in water.

Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 1: Method using freshly prepared bacteria

GSO ISO/TS 23893-2:2013 水质 鱼类的化学分解和生理测量 第2部分：乙氧基沙芬 O-去乙基 (EROD) 的测定

This part of ISO 23893 specifies a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method. It applies to fish that are sampled in their natural environment (fresh water or salt water) or exposed to substances or effluents in a laboratory. This method is applicable for EROD values greater than or equal to 1 pmol/(min·mg) of proteins. A higher sensitivity may be achieved by using a cell (test tube) procedure

Water quality - Biochemical and physiological measurements on fish - Part 2: Determination of ethoxyresorufin-O-deethylase (EROD)

GSO ISO 10712:2013 水质 恶臭假单胞菌生长抑制试验（恶臭假单胞菌细胞增殖抑制试验）

This International Standard specifies a test method for determining the inhibitory effect of surface, ground and waste water on Pseudomonas putida. This method is not suitable for highly coloured test samples, or samples containing undissolved or volatile materials or substances which react with the nutrient solution, or which undergo changes during the test (for example by precipitation, or biochemical or photochemical degradation) and may give false results and/or impair the reproducibility. The method is also suitable for testing substances soluble in water (see annex A).

Water quality -- Pseudomonas putida growth inhibition test (Pseudomonas cell multiplication inhibition test)

GSO ISO 21427-2:2013 水质 通过微核诱导测量评价遗传毒性 第 II 部分：使用 V79 细胞株的混合计数法

This part of ISO 21427 specifies a method for the determination of genotoxicity of water and waste water using a mammalian in vitro test which detects damage, induced by water-soluble substances, to the chromosomes or the mitotic apparatus of V79 cells from the Chinese hamster. The micronucleus test allows the identification of substances that cause cytogenetic damage which results in the formation of micronuclei containing lagging chromosome fragments and/or whole chromosomes. The assay is based on the increase in the frequency of micronucleated cells after incubation with and without metabolic activation

Water quality -- Evaluation of genotoxicity by measurement of the induction of micronuclei -- Part 2: Mixed population method using the cell line V79

GSO ISO 10710:2013 水质 微咸水和微咸水对大型藻类 Kermium Teniocorne 的生长抑制测试

This International Standard specifies a method for the determination of the inhibition of growth of the macroalga Ceramium tenuicorne by substances and mixtures contained in seawater or by waste water with salinities between 4S and 32S. This method is applicable to substances that are easily soluble in water. NOTE With modifications as described in ISO 14442[4] and ISO 5667-16[2], the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metals, waste water, marine water samples, and elutriates of sediments can be tested

Water quality - Growth inhibition test with the marine and brackish water macroalga Ceramium tenuicorne

GSO ISO 23893-1:2013 水质 鱼类的化学分解和生理测量 第1部分：鱼类采样、处理和保存

This part of ISO 23893 provides guidance on how to sample fish for determination of biochemical and physiological characteristics, such as the composition and enzyme activities of blood, liver, muscle and other tissues in order to asses the health of fish in the field as well as in the laboratory. The biochemical and physiological variables used for this purpose are often called biomarkers. This part of ISO 23893 includes recommendations and methods for: obtaining a site-specific sample of a representative number of fish; sampling fish tissues in the field and in the laboratory; and handling and preservation of samples prior to analysis of biochemical and physiological variables

Water quality - Biochemical and physiological measurements on fish - Part 1: Sampling of fish, handling and preservation of samples

GSO ISO 7827:2013 水质 水介质中有机化合物“快速”和“最大”好氧生物降解的评估 溶解有机碳（DOC）分析方法

This International Standard specifies a method for the evaluation of the “ready” and “ultimate” biodegradability of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, this International Standard also gives specific definitions for the terms “ready” and “ultimate”. The method applies to organic compounds which are: a) soluble at the concentration used under the conditions of the test [dissolved organic carbon (DOC) concentrations of 10 mg/l to 40 mg/l]; b) non-volatile or having a negligible vapour pressure under the conditions of the test; c) not significantly adsorbable on glass and activated sludge; d) not inhibitory to the test microorganisms at the concentration chosen for the test. The method is not suitable for waste waters, as they usually contain significant amounts of water-insoluble organic carbon, which is not included in DOC measurements.

Water quality -- Evaluation of the "ready", "ultimate" aerobic biodegradability of organic compounds in an aqueous medium -- Method by analysis of dissolved organic carbon (DOC)

EN ISO 10710:2013 水质.海洋和微咸水大型海藻红藻的生长抑制试验

Diese Internationale Norm legt ein Verfahren zur Bestimmung der Wachstumshemmung der Makroalge Ceramium tenuicorne durch im Meerwasser oder Abwasser mit Salinitaten zwischen 4S und 32S enthaltene Substanzen und Mischungen fest. Dieses Verfahren ist fur gut wasserlosliche Substanzen anwendbar. ANMERKUNG Mit Anderungen, wie sie in ISO 14442 und ISO 5667-16 beschrieben sind, konnen auch Hemmwirkungen von schwerloslichen organischen und anorganischen Verbindungen, fluchtigen Bestandteilen, Abwassern, Meerwasserproben und Eluaten von Sedimenten getestet werden.

Water quality - Growth inhibition test with the marine and brackish water macroalga Ceramium tenuicorne

GSO ISO 9509:2013 水质 毒性测试 以评估活性污泥中微生物的硝化抑制作用

This International Standard specifies a method for assessing the short-term inhibitory effect of waters, waste waters or test substances on nitrifying bacteria in activated sludge. The inhibitory effect is estimated over an exposure period of usually 3 h or up to 24 h with weakly nitrifying sludge. The method is applicable to nitrifying activated sludge derived from domestic and synthetic sewage and also to sludges from industrial and mixed domestic and industrial waste waters. The nitrifying activity of the sludge is verified by testing in the presence and absence of a specific inhibitor (e.g. N-allylthiourea; see Annex A). If the nitrification rate is within a suitable range for the test, i.e. 2 mg of nitrogen per gram of suspended solid and hour to 6,5 mg of nitrogen per gram of suspended solids and hour, the sludge may be used directly. If not, adjustments are necessary (see Clause 9). The method is applicable to water-soluble, non-volatile chemicals, and to waste waters Sludges from different sources respond differently to a given concentration of an inhibitor mainly due to reaction between the inhibitor and components of the sludge. This results in a partial neutralisation of the toxic effect. Also, since the test lasts only hours, any inhibitory effects may diminish or increase over a longer period, e.g. in the continuous activated sludge system (see ISO 5667-16).

Water quality - Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms

GSO ISO 20666:2013 水质 48小时内Brychonius cleissoflores慢性毒性的测定

This International Standard specifies a method for the determination of the chronic toxicity to rotifer Brachionus calyciflorus, based on population growth inhibition in 48 h. The method is applicable to: a) chemical substances which are soluble or which can be maintained as stable suspensions or dispersions under the conditions of the test; b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration or centrifugation; c) fresh waters; d) aqueous extracts. This International Standard is not applicable to the testing of unstable chemicals (hydrolysing, absorbing, etc.) in water unless exposure concentration is measured, nor to the testing of aquatic samples from the estuarine or marine environment

Water quality -- Determination of the chronic toxicity to Brachionus calyciflorus in 48 h

GSO ISO 10253:2013 水质 中肋骨条藻和三角褐指藻抑制海藻生长的试验

This International Standard specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema costatum and Phaeodactylum tricornutum by substances and mixtures contained in sea water. The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium. NOTE With modifications, as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metal compounds, effluents, marine water samples and elutriates of sediments can be tested.

Water quality -- Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum

GSO ISO 9439:2013 水质 水介质中有机化合物最大需氧生物降解评价 二氧化碳产生试验

This International Standard specifies a method, by determination of carbon dioxide (CO2), for the evaluation in an aqueous medium of the ultimate biodegradability of organic compounds at a given concentration by aerobic microorganisms. The method applies to organic compounds which are: a) water-soluble under the conditions of the test, in which case removal of DOC may be determined as additional information (see annex D); b) poorly water-soluble under the conditions of the test, in which case special measures may be necessary to achieve good dispersion of the compound (see, for example, ISO 10634); c) non-volatile or which have a negligible vapour pressure under the conditions of the test; NOTE For volatile substances use for example ISO 9408 or ISO 14593. d) not inhibitory to the test microorganisms at the concentration chosen for the test. NOTE The presence of inhibitory effects can be determined as specified in 8.3, or by using any other method for determining the inhibitory effect of a compound on bacteria (see, for example, ISO 8192).

Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Carbon dioxide evolution test

GSO ISO/TR 11044:2013 水质 藻类生长抑制测试的科学和技术方面

This Technical Report discusses scientific and technical aspects that have been considered in connection with the development of batch algal growth inhibition test procedures specified in ISO 8692, for freshwater, and ISO 10253, for marine waters. Previously unpublished results of experiments performed at the Norwegian Institute for Water Research (NIVA) have been included to demonstrate various aspects

Water quality -- Scientific and technical aspects of batch algae growth inhibition tests

GSO ISO 11348-2:2013 水质水样对甲壳类弧菌发光抑制作用的测定（发光细菌试验） 第2部分：使用干液细菌的方法

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using liquid-dried bacteria. This method is applicable to: — waste water; — aqueous extracts and leachates; — fresh water (surface water and ground water); — sea water and brackish water; — eluates of sediment (fresh water, brackish water and sea water); — pore water; — single substances, diluted in water.

Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 2: Method using liquid-dried bacteria

GSO ISO 21427-1:2013 水质 使用微核诱导测量评估遗传毒性 第1部分：使用两栖动物幼虫评估遗传毒性

This part of ISO 21427 specifies a method for assessing genotoxicity using Amphibia larvae (Xenopus laevis and Pleurodeles waltl). The provisions specific to Pleurodeles are described in Annex A. The method is applicable to: ⎯ aqueous effluents; ⎯ aqueous leachates; ⎯ eluates of soils; ⎯ eluates of industrial waste; ⎯ eluates of sludges from sewage treatment; ⎯ surface and ground water; ⎯ water-soluble substances.

Water quality -- Evaluation of genotoxicity by measurement of the induction of micronuclei -- Part 1: Evaluation of genotoxicity using amphibian larvae