13.060.70 水的生物学特性的检验 标准查询与下载

ISO 16191:2013 水质.狐尾藻(Myriopghllum aquaticum)生长行为过程中沉积物毒性作用测定

This International Standard specifies a method for determining the toxicity of environmental samples on the growth of Myriophyllum aquaticum. The method described is applicable to natural fresh water sediment and artificial sediment.

Water quatity-Determination of the toxic effect of sediment on the growth behaviour of Myriopghllum aquaticum

LST EN ISO 7827:2013 水质 评估水性介质中有机化合物的“准备就绪”、“最终”需氧生物降解能力 通过分析溶解有机碳（DOC）的方法（ISO 7827:2010）

Water quality - Evaluation of the "ready", "ultimate" aerobic biodegradability of organic compounds in an aqueous medium - Method by analysis of dissolved organic carbon (DOC) (ISO 7827:2010)

BS ISO 23893-3:2013 水质.鱼的生物化学和生理测量.卵黄蛋白原的测定

Water quality. Biochemical and physiological measurements on fish. Determination of vitellogenin

GSO ISO 20079:2013 水质 评估水和废水成分对浮萍的毒性影响 浮萍生长抑制试验

This International Standard specifies a method for the determination of the growth-inhibiting response of duckweed (Lemna minor) to substances and mixtures contained in water, treated municipal wastewater and industrial effluents.

Water quality – Determination of the toxic effect of water constituents and waste water on duckweed (Lemna minor) – Duckweed growth inhibition test

GSO ISO 20666:2013 水质 48小时内Brychonius cleissoflores慢性毒性的测定

This International Standard specifies a method for the determination of the chronic toxicity to rotifer Brachionus calyciflorus, based on population growth inhibition in 48 h. The method is applicable to: a) chemical substances which are soluble or which can be maintained as stable suspensions or dispersions under the conditions of the test; b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration or centrifugation; c) fresh waters; d) aqueous extracts. This International Standard is not applicable to the testing of unstable chemicals (hydrolysing, absorbing, etc.) in water unless exposure concentration is measured, nor to the testing of aquatic samples from the estuarine or marine environment

Water quality -- Determination of the chronic toxicity to Brachionus calyciflorus in 48 h

GSO ISO 7346-3:2013 水质 物质对淡水鱼的急性致死毒性的测定[Brachydanio rerio Hamilton-Buchanan（Teleostei Cyprinidae）] 第3部分：流通法

This part of ISO 7346 specifies a flow-through method for the determination of the acute lethal toxicity of stable, non-volatile, single substances, soluble in water under specified conditions, to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae) — common name, zebra fish] in water of a specified quality. The method is applicable for assigning, for each test substance, broad categories of acute lethal toxicity to Brachydanio rerio under the test conditions. The results are insufficient by themselves to define water quality standards for environmental protection. The method is also applicable when using certain other species of freshwater fish as the test organism1). The method may be adapted for use with other freshwater fish and marine and brackish water fish with appropriate modification of the test conditions, particularly with respect to the quantity and quality of the dilution water and the temperature.

Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 3: Flow-through method

GSO ISO 9509:2013 水质 毒性测试 以评估活性污泥中微生物的硝化抑制作用

This International Standard specifies a method for assessing the short-term inhibitory effect of waters, waste waters or test substances on nitrifying bacteria in activated sludge. The inhibitory effect is estimated over an exposure period of usually 3 h or up to 24 h with weakly nitrifying sludge. The method is applicable to nitrifying activated sludge derived from domestic and synthetic sewage and also to sludges from industrial and mixed domestic and industrial waste waters. The nitrifying activity of the sludge is verified by testing in the presence and absence of a specific inhibitor (e.g. N-allylthiourea; see Annex A). If the nitrification rate is within a suitable range for the test, i.e. 2 mg of nitrogen per gram of suspended solid and hour to 6,5 mg of nitrogen per gram of suspended solids and hour, the sludge may be used directly. If not, adjustments are necessary (see Clause 9). The method is applicable to water-soluble, non-volatile chemicals, and to waste waters Sludges from different sources respond differently to a given concentration of an inhibitor mainly due to reaction between the inhibitor and components of the sludge. This results in a partial neutralisation of the toxic effect. Also, since the test lasts only hours, any inhibitory effects may diminish or increase over a longer period, e.g. in the continuous activated sludge system (see ISO 5667-16).

Water quality - Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms

GSO ISO/TR 11044:2013 水质 藻类生长抑制测试的科学和技术方面

This Technical Report discusses scientific and technical aspects that have been considered in connection with the development of batch algal growth inhibition test procedures specified in ISO 8692, for freshwater, and ISO 10253, for marine waters. Previously unpublished results of experiments performed at the Norwegian Institute for Water Research (NIVA) have been included to demonstrate various aspects

Water quality -- Scientific and technical aspects of batch algae growth inhibition tests

GSO ISO 16221:2013 水质 海洋环境生物降解评估指南

This International Standard specifies five methods for determining the ultimate aerobic biodegradability of organic compounds in the marine environment by aerobic microorganisms in static aqueous test systems. Standard degradation methods developed for testing in fresh water are modified and adapted to marine conditions. These methods are the DOC die-away test (ISO 7827), the closed bottle test (ISO 10707), the two-phase closed bottle test (ISO 10708), the CO2 evolution test (ISO 9439) and the CO2 headspace test (ISO 14593). The methods apply to organic compounds which a) are water-soluble under the conditions of the test used; b) are poorly water-soluble under the conditions of the test used, in which case special measures may be necessary to achieve good dispersion of the compound (see for example, ISO 10634); c) are volatile, provided that an appropriate test with suitable conditions is used; d) are not inhibitory to the test microorganisms at the concentration chosen for the tests. The presence of inhibitory effects can be determined as specified in this International Standard.

Water quality – Guidance for determination of biodegradability in the marine environment

GSO ISO/TS 20281:2013 水质 生态毒理学数据统计解释指南

This Technical Specification offers guidance on statistical methods used for the analysis of data of standardized ecotoxicity tests. It focuses on statistical methods for obtaining statistical estimates of parameters in current and future use, e.g. ECx (LCx), NOEC, NEC. The methods described are intended to cover laboratory ecotoxicity tests (aquatic, sediment and/or terrestrial tests), and may also be relevant for other toxicity tests. The main objective of this Technical Specification is to provide practical guidance on how to analyse the observations from ecotoxicity tests. Hypothesis testing, concentration-response modelling and biology-based modelling are discussed for the different data types (quantal, continuous and discrete data, corresponding to mortality, growth or reproduction). In addition, some guidance on experimental design is given. Although the main focus is on giving assistance to the experimentalist, a secondary aim is to help those who are responsible for evaluating toxicity tests. Finally, the document may be helpful in developing new toxicity test guidelines by giving information on experimental design and statistical analysis issues.

Water quality -- Guidance on statistical interpretation of ecotoxicity data

GSO ISO 10253:2013 水质 中肋骨条藻和三角褐指藻抑制海藻生长的试验

This International Standard specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema costatum and Phaeodactylum tricornutum by substances and mixtures contained in sea water. The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium. NOTE With modifications, as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metal compounds, effluents, marine water samples and elutriates of sediments can be tested.

Water quality -- Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum

GSO ISO 9887:2013 水质 水介质中有机化合物好氧生物降解的评价 半连续活性污泥（SCAS）法

This International Standard specifies a method for the evaluation of the biodegradability ("ultimate" or "primary") of organic compounds. The conditions described in this International Standard are much more favorable for biodegradation than those specified in the methods for biodegradability described in ISO 7827, ISO 9408 and ISO 9439. The method applies to organic compounds which are a) soluble at the concentration used under the test conditions; b) non-volatile, or which have a negligible vapour pressure under the test conditions; c) not lost by foaming from the test solution; d) not significantly adsorbable on glass and activated sludge; e) not inhibitory to the test micro-organisms at the concentration chosen for the test. Inhibitory effects can be determined by using a suitable test method (e.g. see ISO 8192). If the test compound is toxic, the test concentration has to be lower or a pre-exposed inoculum can be used. NOTE 1 Additionally, or alternatively, the semicontinuous activated sludge (SCAS) units may be used to provide sludge exposed to the test compound, in order to see whether the sludge becomes adapted, to be used as inocula in other biodegradation tests.

Water quality - Evaluation of the aerobic biodegradability of organic compounds in an aqueous medium - Semi-continuous activated sludge method (SCAS)

GSO ISO 10712:2013 水质 恶臭假单胞菌生长抑制试验（恶臭假单胞菌细胞增殖抑制试验）

This International Standard specifies a test method for determining the inhibitory effect of surface, ground and waste water on Pseudomonas putida. This method is not suitable for highly coloured test samples, or samples containing undissolved or volatile materials or substances which react with the nutrient solution, or which undergo changes during the test (for example by precipitation, or biochemical or photochemical degradation) and may give false results and/or impair the reproducibility. The method is also suitable for testing substances soluble in water (see annex A).

Water quality -- Pseudomonas putida growth inhibition test (Pseudomonas cell multiplication inhibition test)

GSO ISO 21427-2:2013 水质 通过微核诱导测量评价遗传毒性 第 II 部分：使用 V79 细胞株的混合计数法

This part of ISO 21427 specifies a method for the determination of genotoxicity of water and waste water using a mammalian in vitro test which detects damage, induced by water-soluble substances, to the chromosomes or the mitotic apparatus of V79 cells from the Chinese hamster. The micronucleus test allows the identification of substances that cause cytogenetic damage which results in the formation of micronuclei containing lagging chromosome fragments and/or whole chromosomes. The assay is based on the increase in the frequency of micronucleated cells after incubation with and without metabolic activation

Water quality -- Evaluation of genotoxicity by measurement of the induction of micronuclei -- Part 2: Mixed population method using the cell line V79

GSO ISO 11348-3:2013 水质水样对十字花科弧菌发光抑制效果的测定（发光细菌试验） 第3部分：使用冻干细菌的方法

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using freeze-dried bacteria. This method is applicable to: — waste water; — aqueous extracts and leachates; — fresh water (surface and ground water); — sea and brackish water; — eluates of sediment (freshwater, brackish and sea water); — pore water; — single substances, diluted in water.

Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 3: Method using freeze-dried bacteria

GSO ISO 18749:2013 水质 活性污泥材料的吸附 使用特定分析方法进行批量测试

This International Standard specifies a screening test method for the determination of the degree of adsorption of substances on to the activated sludge or primary sludge in a waste water treatment plant. The conditions described in this International Standard normally correspond to the optimum conditions for the adsorption to occur at the chosen activated-sludge concentration and water hardness during the test period. The method applies to substances for which an analytical method with sufficient accuracy is available and which, under the conditions of the test and at the test concentration used, a) are water-soluble; b) or, if only slightly water-soluble, allow sufficiently stable suspensions, dispersions or emulsions to be prepared; c) are not significantly removed from the test solution during the test by known abiotic processes such as stripping or foaming; d) do not deflocculate activated sludge; e) are not readily biodegradable (for a discussion of biodegradability, see ISO/TR 15462). An important parameter that can influence the reliability of the test results is the stability of the test compound during the test. If no information on the stability is available, it is recommended that this be checked before the test. If any transformation (e.g. due to hydrolysis) is observed, it is recommended that the degree of adsorption of the transformation products be determined, if possible. Since biodegradability of the test compound may also lead to an incorrect assessment of the degree of adsorption, it is recommended that the biodegradability be investigated in advance using standard biodegradation tests which are preferably based on oxygen consumption or on carbon dioxide production and in which adsorption has no influence on the test result. If biodegradation cannot be excluded, sterilized sludge may be used (see Clause 7). There is generally no need to carry out adsorption tests on substances which are readily biodegradable as they are sufficiently removed biologically in waste water treatment plants. Substances which are easily adsorbed on activated sludge in waste water treatment plants are preferably removed by adsorbing them in sludge digesters and degrading them anaerobically. For such substances, high adsorption may be a reason for carrying out anaerobic biodegradation tests. An overview of standardized biodegradation tests is given in ISO/TR 15462. The test compound concentrations used in this method are usually very low and therefore no negative effects are to be expected on the capacity of the activated sludge to adsorb even toxic test compounds. When there is any doubt, it is recommended that microscopic investigations of the flocs and suitable toxicity tests such as that specified in ISO 8192 be carried out.

Water quality – Adsorption of substances on activated sludge – Batch test using specific analytical methods

EN ISO 10710:2013 水质.海洋和微咸水大型海藻红藻的生长抑制试验

Diese Internationale Norm legt ein Verfahren zur Bestimmung der Wachstumshemmung der Makroalge Ceramium tenuicorne durch im Meerwasser oder Abwasser mit Salinitaten zwischen 4S und 32S enthaltene Substanzen und Mischungen fest. Dieses Verfahren ist fur gut wasserlosliche Substanzen anwendbar. ANMERKUNG Mit Anderungen, wie sie in ISO 14442 und ISO 5667-16 beschrieben sind, konnen auch Hemmwirkungen von schwerloslichen organischen und anorganischen Verbindungen, fluchtigen Bestandteilen, Abwassern, Meerwasserproben und Eluaten von Sedimenten getestet werden.

Water quality - Growth inhibition test with the marine and brackish water macroalga Ceramium tenuicorne

GSO ISO 9888:2013 水质 水介质中有机化合物最大需氧生物降解的评估 静态测试（Zahn-Velence 方法）

This International Standard specifies a method for the evaluation in aqueous medium of the ultimate biodegradability and, as additional information, the primary biodegradability and the total elimination from water, of organic compounds at a given concentration by aerobic microorganisms. The conditions described in this International Standard normally correspond to optimal conditions for allowing the maximum value of biodegradation to occur with the chosen inoculum in the test time. These conditions may even be more favourable than in full-scale wastewater treatment plants, especially if their hydraulic retention time, sludge age or the adaptation of the activated sludge is not optimal. The method applies to organic compounds which are a) water-soluble at the concentration used under the test conditions and not expected to be transformed to insoluble metabolites if biodegradation and not elimination only shall be determined; b) nonvolatile, or which have a negligible vapour pressure under the test conditions; c) not lost by foaming from the test solution; d) not inhibitory to the test microorganisms at the concentration chosen for the test. Inhibitory effects can be determined using a suitable test method (e.g. see ISO 8192). If the test compound is toxic, the test concentration must be lowered, or a pre-exposed inoculum can be used. This International Standard is also applicable to the measurement of biodegradation and elimination of dissolved organic compounds in wastewater (also called "test compound" in the method). NOTE If more information is required to predict the behaviour of test compounds or wastewater in a treatment plant, a simulation test (e.g. the activated sludge simulation test ISO 11733) should be performed. For appropriate use of this method and for alternative biodegradation methods, see ISO 15462.

Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)

GSO ISO 9439:2013 水质 水介质中有机化合物最大需氧生物降解评价 二氧化碳产生试验

This International Standard specifies a method, by determination of carbon dioxide (CO2), for the evaluation in an aqueous medium of the ultimate biodegradability of organic compounds at a given concentration by aerobic microorganisms. The method applies to organic compounds which are: a) water-soluble under the conditions of the test, in which case removal of DOC may be determined as additional information (see annex D); b) poorly water-soluble under the conditions of the test, in which case special measures may be necessary to achieve good dispersion of the compound (see, for example, ISO 10634); c) non-volatile or which have a negligible vapour pressure under the conditions of the test; NOTE For volatile substances use for example ISO 9408 or ISO 14593. d) not inhibitory to the test microorganisms at the concentration chosen for the test. NOTE The presence of inhibitory effects can be determined as specified in 8.3, or by using any other method for determining the inhibitory effect of a compound on bacteria (see, for example, ISO 8192).

Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Carbon dioxide evolution test

GSO ISO 14442:2013 水质 难溶物质、挥发性化合物、矿物质和剩余水藻类生长抑制剂测试指南

This International Standard provides procedures, not covered by the methods described in ISO 8692 and ISO 10253, for testing difficult substances for inhibition of algal growth. The main subjects covered by the guideline are the methods for preparing the test substance for testing and the procedures needed to carry out an appropriate test. The following test substances are covered by this guideline: a) poorly soluble pure organic compounds; b) poorly soluble mixtures of organic substances; c) poorly soluble inorganic materials; d) volatile substances; e) waste waters and environmental samples containing water and sediments; f) coloured and/or turbid samples; g) compounds of heavy metals. The following methods of addition are covered: ⎯ direct; ⎯ dispersion; ⎯ water-soluble and water-accommodated fractions. Some guidelines related to the analytical procedures and to the interpretation of the results have been included. References to documents describing the background for the testing of difficult substances are given in the Bibliography.

Water quality -- Guidelines for algal growth inhibition tests with poorly soluble materials, volatile compounds, metals and waste water