13.060.70 水的生物学特性的检验 标准查询与下载

GSO ISO 14592-2:2013 水质 低浓度有机化合物好氧生物降解性评价 第2部分：带有附着生物量的连续流河流模型

This part of ISO 14592 specifies a method for evaluating the biodegradability of organic test compounds by aerobic microorganisms in natural waters by means of a continuous flow river model with attached biomass. This part of ISO 14592 is applicable to organic test compounds present in lower concentrations than those of natural carbon substrates also present in the system. Under these conditions, the test compounds serve as a secondary substrate and the kinetics for biodegradation would be expected to be first order (“non-growth” kinetics). This part of ISO 14592 is applicable to organic test compounds, which under the conditions of the test and at the chosen test concentration, are:  water soluble;  quantitatively detectable with appropriate analytical methods or available in radiolabelled form;  non-volatile from aqueous solution (e.g. Henry's law constant < 1 Pa⋅m3/mole);  not significantly adsorbed;  not photolyzed;  not inhibitory to the microorganisms of the test system. The test is not recommended for use as proof of ultimate biodegradability (mineralization) which is better assessed using other standardized tests (see ISO/TR 15462).

Water quality -- Evaluation of the aerobic biodegradability of organic compounds at low concentrations -- Part 2: Continuous flow river model with attached biomass

GSO ISO 11348-2:2013 水质水样对甲壳类弧菌发光抑制作用的测定（发光细菌试验） 第2部分：使用干液细菌的方法

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using liquid-dried bacteria. This method is applicable to: — waste water; — aqueous extracts and leachates; — fresh water (surface water and ground water); — sea water and brackish water; — eluates of sediment (fresh water, brackish water and sea water); — pore water; — single substances, diluted in water.

Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 2: Method using liquid-dried bacteria

GSO ISO 7346-3:2013 水质 物质对淡水鱼的急性致死毒性的测定[Brachydanio rerio Hamilton-Buchanan（Teleostei Cyprinidae）] 第3部分：流通法

This part of ISO 7346 specifies a flow-through method for the determination of the acute lethal toxicity of stable, non-volatile, single substances, soluble in water under specified conditions, to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae) — common name, zebra fish] in water of a specified quality. The method is applicable for assigning, for each test substance, broad categories of acute lethal toxicity to Brachydanio rerio under the test conditions. The results are insufficient by themselves to define water quality standards for environmental protection. The method is also applicable when using certain other species of freshwater fish as the test organism1). The method may be adapted for use with other freshwater fish and marine and brackish water fish with appropriate modification of the test conditions, particularly with respect to the quantity and quality of the dilution water and the temperature.

Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 3: Flow-through method

GSO ISO 20665:2013 水质 Cyropodin Diopie 慢性毒性的估计

This International Standard specifies a method for the determination of the chronic toxicity to Ceriodaphnia dubia (Cladocera, Crustacea), based on reproduction inhibition after (7 ± 1) d. The method is applicable to: a) chemical substances which are soluble or which can be maintained as stable suspensions or dispersions under the conditions of the test; b) industrial or sewage effluents, if appropriate after decantation, filtration or centrifugation; c) fresh waters; d) aqueous extracts. This International Standard is not applicable to the testing of aquatic samples from the estuarine or marine environment.

Water quality -- Determination of chronic toxicity to Ceriodaphnia dubia

GSO ISO 11348-1:2013 水质水样对甲壳类弧菌发光抑制效果的测定（发光细菌试验） 第1部分：新鲜菌的使用方法

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using freshly prepared bacteria. This method is applicable to: — waste water; — aqueous extracts and leachates; — fresh water (surface and ground water); — sea and brackish water; — eluates of sediment (fresh water, brackish and sea water); — pore water; — single substances, diluted in water.

Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 1: Method using freshly prepared bacteria

ISO 23893-3:2013 水质.鱼的生物化学和生理测量.第3部分:卵黄蛋白原的测定

This part of ISO 23893 specifies a method for measuring vitellogenin (Vtg) concentrations in a fish plasma sample employing an enzyme-linked immunosorbent assay (ELISA) method. It applies to fish that are sampled in the environment (fresh, estuarine or salt water) and to fish exposed to substances or effluents in a laboratory. The method is quantitative when using Vtg antibodies and a Vtg standard well characterized with the species of choice.

Water quality - Biochemical and physiological measurements on fish - Part 3: Determination of vitellogenin

ASTM E2591-07(2013) 用两栖动物进行全部沉积物毒性试验的标准指南

1.1 This standard covers procedures for obtaining laboratory data concerning the toxicity of test material (for example, sediment or hydric soil (that is, a soil that is saturated, flooded, or ponded long enough during the growing season to develop anaerobic (oxygen-lacking) conditions that favor the growth and regeneration of hydrophytic vegetation)) to amphibians. This test procedure uses larvae of the northern leopard frog (Rana pipiens). Other anuran species (for example, the green frog (Rana clamitans), the wood frog (Rana sylvatica), the American toad (Bufo americanus)) may be used if sufficient data on handling, feeding, and sensitivity are available. Test material may be sediments or hydric soil collected from the field or spiked with compounds in the laboratory. 1.2 The test procedure describes a 10-d whole sediment toxicity test with an assessment of mortality and selected sublethal endpoints (that is, body width, body length). The toxicity tests are conducted in 300 to 500-mL chambers containing 100 mL of sediment and 175 mL of overlying water. Overlying water is renewed daily and larval amphibians are fed during the toxicity test once they reach Gosner stage 25 (operculum closure over gills). The test procedure is designed to assess freshwater sediments, however, R. pipiens can tolerate mildly saline water (not exceeding about 2500 mg Cl/L, equivalent to a salinity of about 4.1 when Na+ is the cation) in 10-d tests, although such tests should always include a concurrent freshwater control. Alternative test durations and sublethal endpoints may be considered based on site-specific needs. Statistical evaluations are conducted to determine whether test materials are significantly more toxic than the laboratory control sediment or a field-collected reference sample(s). 1.3 Where appropriate, this standard has been designed to be consistent with previously developed methods for assessing sediment toxicity to invertebrates (for example, Hyalella azteca and Chironomus dilutus toxicity tests) described in the United States Environmental Protection Agency (USEPA, (1))2 freshwater sediment testing guidance, Test Methods E1367 and E1706, and Guides E1391, E1525, E1611, and E1688. Tests extending to 10 d or beyond, and including sublethal measurements such as growth, are considered more effective in identifying chronic toxicity and thus delineating areas of moderate contamination (1-3). 1.4 Many historical amphibian studies, both water and sediment exposure, have used tests of shorter duration (5 days or less) (for example, 4-7) and, although both survival and sublethal endpoints were often assessed, there is substantive evidence that tests of longer duration are likely to be more sensitive to some contaminants (8, 9). Research performed to develop and validate this test protocol included long-term (through metamorphosis) investigations and other researchers have also conducted long-duration tests with anurans (7-11). In the development of these procedures, an attempt was made to balance the needs of a practical assessment with the importance of assessing longer-term effects so that the results will demonstrate the needed accuracy and precision. The most recent sediment toxicity testing protocols for invertebrates have encompassed longer duration studies which allow the measurement of reproductive endpoints (1, 12). Such tests, because of increased sensitivity of the sublethal endpoints, may also be helpful in evaluating toxicity. Full life-cycle studies with anurans (including reproduction) are usually not feasible from either a technical or monetary standpoint. However, if sitespecific information indicates that the contaminants present are likely to affect other endpoints (including teratogenicity), then the duration of the toxicity test may be increased through metamorphosis or additional sublethal endpoints may be measured (for example, impaired behavior, deformities, time-tometamorphosis). The possible inclusion of these endpoints and extension of test length should be considered during development of the project or study plan (see 8.1.1). 1.5 The methodology presented in this standard was developed under a Department of Defense (DoD) research program and presented in a guidance manual for risk assessment staff 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved March 1, 2013. Published March 2013. Originally approved in 2007. Last previous edition approved in 2007 as E2591–07. DOI: 10.1520/E2591-07R13. 2 The boldface numbers in parentheses refer to the list of references at the end of this standard. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States 1 and state/federal regulators involved in the review and approval of risk assessment work plans and reports (13). To develop this method, a number of tests with spiked sediment tests were conducted (13, 14). Since development of the methodology it has been used operationally to evaluate fieldcollected sediments from several state and federal environmental sites (15, 16). For most of these studies the preferred test organisms, Rana pipiens, was used. At a lead-contaminated state-led site, operated by the Massachusetts Highway Department, Xenopus laevis(African clawed frog) was used in the sediment test system because of availability problems with Rana pipiens (17), The test method was also used to evaluate sediment toxicity at a cadmium-contaminated USEPA Region 4-led site in Tennessee (18). The methodology was used to help characterize potential effects of contaminants on amphibians and to help develop preliminary remedial goals, if warranted. All tests evaluated survival and growth effects after 10 d of exposure in accordance with the methods presented in this standard. 1.6 The use of larval amphibians to assess environmental toxicity is not novel. Researchers have used tadpoles to examine toxicity of metals and organic compounds. Most of these studies have been through water exposure, usually in a manner similar to fish or invertebrate exposure as described in Guide E729 (19-29). Fewer studies have focused on exposure of anuran larvae to sediments, and the methods employed vary widely, from in situ enclosures (30) to laboratory tests using variable exposure conditions and organism ages (4, 8, 31-33). No studies were identified that used the same test conditions as described in this standard. However, several laboratory-based evaluations of sediment effects on amphibians are described in the following subsections. 1.6.1 Sediment toxicity tests conducted in the laboratory with amphibians were performed over a range of test durations from 4 d (4, 31, Guide E1439-98 Appendix X2) to 12 d (33) and through metamorphosis (8, 32). Sediment toxicity tests with anurans native to North America were started with larval tadpoles between Gosner stages 23 and 25 (8, 32, 33). Test temperatures were between 21 and 23°C and feeding began after tadpoles reached Gosner stage 25. Food sources were Tetramin™ (8), boiled romaine lettuce (32), or boiled romaine lettuce and dissipated rabbit food pellets (33). Tests were conducted in static renewal mode with water replacements conducted at varying rates (daily (31, 33), weekly (8), every 3 to 5 d (32)). Test design (number of replicates, test vessel size, number of organisms per replicate) varied depending on the objective of the study with several tests conducted in aquaria (32), large bins (8), or swimming pools (33). Endpoints evaluated at test termination included survival (4, 8, 31-33), growth (8, 31-33), bioaccumulation of metals (8), developmental rates (8, 32), deformities (31, 32), swimming speed (33) and foraging activity levels (32). 1.6.2 To assess the effect of direct contact with the sediments containing PCBs, Savage et al. (32) exposed larval tadpoles (Gosner stage 23 to 25; wood frogs (R. sylvatica)) to field-collected sediments under conditions that allowed both direct contact with the sediment and separation from the sediment with a 500 µm mesh barrier. The study found that lethal and sublethal effects on tadpoles observed through metamorphosis were more pronounced when direct contact with the sediment was allowed. The test conditions described in this standard allow tadpoles to maintain direct contact with the sediment. 1.6.3 Sediment toxicity testing with Xenopus laevis has focused on evaluating the developmental effects of sediment extracts, as opposed to whole sediments, on frog embryos. Methods have been developed which expose blastula stage embryos to sediment by enclosing the embryos in a Teflon mesh insert that rests over the top of the sediment in the sediment–water interface region (31, Guide E1439-98 Appendix X2). These studies are conducted evaluate survival, growth, and physical malformations of the embryos after a 4-d exposure period. The test conditions described in this standard allow more direct contact with the sediment, using older test organisms, and a longer exposure duration. 1.7 Sediment toxicity tests are an effective means for evaluating the impact of sediment contamination on amphibians in a multiple lines of evidence paradigm. The evaluation is most powerful when toxicity testing sampling stations are co-located with sediment analytical chemistry samples and ecological surveys, allowing for a detailed evaluation of the co-occurring data in the ecological risk assessment. The spatial and temporal co-location of toxicity testing and analytical samples is particularly important for establishing contaminantspecific effects and assessing contaminant bioavailability. 1.8 In order for a sediment toxicity test to be sensitive it must be of sufficient duration to measure potential toxicity and it must be conducted during the appropriate developmental stage of the test organism’s life cycle. Using recently hatched tadpoles and conducting the sediment exposure test for 10 d to allow the evaluation of growth endpoints meets both of these sensitivity requirements. 1.9 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.10 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians

LST EN ISO 6341:2013 水质-大型溞（Cladocera, Crustacea）迁移率抑制的测定-急性毒性试验（ISO 6341:2012）

Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test (ISO 6341:2012)

DS/EN ISO 7827:2013 水质 水性介质中有机化合物“准备就绪”，“最终”需氧生物降解能力的评估 溶解有机碳（DOC）分析法

ISO 7827:2010 specifies a method for the evaluation of the "ready" and "ultimate" biodegradability of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, ISO 7827:2010 also gives specific definitions for the te

Water quality - Evaluation of the "ready", "ultimate" aerobic biodegradability of organic compounds in an aqueous medium - Method by analysis of dissolved organic carbon (DOC)

DS/EN ISO 6341:2013 水质. 大型溞（Cladocera, Crustacea）迁移率抑制的测定. 急性毒性试验

This International Standard specifies a method for the determination of the acute toxicity to Daphnia magna Straus (Cladocera, Crustacea).This method is applicable to:— chemical substances which are soluble under the conditions of the test, or can be main

Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test

DIN EN ISO 6341:2013 水质.大型水蚤(Daphnia magna Straus枝角目,甲壳纲类)的机动性抑制影响测定.急性中毒试验(ISO 6341-2012).德文版本EN ISO 6341-2012

This International Standard specifies a method for the determination of the acute toxicity to Daphnia magna Straus (Cladocera,Crustacea). This method is applicable to: -chemical substances which are soluble under the conditions of the test, or can be maintained as a stable suspension or dispersion under the conditions of the test; -industrial or sewage effluents; -treated or untreated waste water; -aqueous extracts and leachates; -fresh water (surface and ground water); -eluates of fresh water sediment; -pore water of fresh water sediment.

Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test (ISO 6341:2012); German version EN ISO 6341:2012

NF T90-301*NF EN ISO 6341:2012 水质 抑制大型水蚤(Daphnia magna Straus)(枝角目Cladocera,甲壳纲Crustacea)活动性的测定 急性毒性试验

Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test

LST EN ISO 10870:2012 水质 淡水底栖大型无脊椎动物采样方法和设备选择指南（ISO 10870:2012）

Water quality - Guidelines for the selection of sampling methods and devices for benthic macroinvertebrates in fresh waters (ISO 10870:2012)

NF T90-707*NF ISO 11350:2012 水质.水和废水基因毒性测定.沙门氏菌/微粒体变异反应试验(艾姆斯氏变异反应试验)

Water quality - Determination of the genotoxicity of water and waste water - Salmonela/microsome fluctuation test (Ames fluctuation test)

LST EN 16161-2012 水质 关于使用体内吸收技术估计海洋和淡水样品中叶绿素-a 浓度的指南

Water quality - Guidance on the use of in vivo absorption techniques for the estimation of chlorophyll-a concentration in marine and fresh water samples

BS EN ISO 6341:2012 水质. 大型蚤 (枝角目, 甲壳纲) 活性抑制力的测定. 急性毒性试验

Water quality. Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea). Acute toxicity test

DIN ISO 10872:2012 水质.沉淀物和土壤样本上的秀丽隐杆线虫(Caenorhabditis elegans,线虫纲Nematoda)的生长、生育和繁殖毒性的测定(ISO 10872-2010)

This International Standard specifies a method for cetermining the toxicity of environmental samples on growth,fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole fresh-water sediment (maximum salinity 5 ‰),soil and waste,as well as to pore water,elutriates and aqueous extracts that were obtained from contaminated sediment,soil and waste.

Water quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2010)

DIN EN ISO 10710:2012 水质.海洋和微咸水大型海藻红藻(Ceramium tenuicorne)的成长抑制测试(ISO 10710-2010).德文版本FprEN 10710-2012

Water quality - Growth inhibition test with the marine and brackish water macroalga Ceramium tenuicorne (ISO 10710:2010); German version FprEN 10710:2012

DIN EN ISO 10870:2012 水质.淡水中水底大型无脊椎动物取样方法和设备筛选导则(ISO 10870-2012).德文版本EN ISO 10870-2012

Water quality - Guidelines for the selection of sampling methods and devices for benthic macroinvertebrates in fresh waters (ISO 10870:2012); German version EN ISO 10870:2012

ISO 6341:2012 水质.大型水蚤(Daphnia magna Straus枝角目,甲壳纲类)的机动性抑制影响测定.急性中毒试验

This International Standard specifies a method for the determination of the acute toxicity to Daphnia magna Straus (Cladocera, Crustacea). This method is applicable to: — chemical substances which are soluble under the conditions of the test, or can be maintained as a stable suspension or dispersion under the conditions of the test; — industrial or sewage effluents; — treated or untreated waste water; — aqueous extracts and leachates; — fresh water (surface and ground water); — eluates of fresh water sediment; — pore water of fresh water sediment.

Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test