07.100.30;67.050 标准查询与下载

DIN CEN/TS 15634-2:2012 食品.用分子生物方法检测食物过敏原.第2部分:芹菜.熟香肠中DNA排序实时监控的定性分析.德文版本CEN/TS 15634-2-2012

Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium graveolens) - Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR; German version CEN/TS 15634-2:2012

PD CEN/TS 15634-2:2012 食品.用分子生物方法检测食物过敏原.第2部分:芹菜.熟香肠中DNA排序实时监控的定性分析

This Technical Specification specifies a method for the qualitative detection of celery (Apium graveolens) in emulsion-type sausages (e. g. Frankfurter, Wiener). Real-time PCR detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF067082) of celery (Apium graveolens). The method has been validated on emulsion-type sausages (Bavarian "Leberk？se") spiked with celery. ForThis purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and 1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds or celery root powder to 1000 mg/kg. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65 ℃ for 60 min.

Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium graveolens) - Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR

DIN EN 15634-1:2009 食品 使用分子生物学方法探测食物过敏原 第1部分:一般考虑

This European Standard provides the overall framework for detection of sequences corresponding to species containing allergens using the polymerase chain reaction (PCR). It relates to the requirements for the specific amplification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified nucleic acid sequence. Guidelines, minimum requirements and performance criteria laid down in the European Standard are intended to ensure that comparable and reproducible results are obtained in different laboratories. This European Standard has been established for food matrices.

Foodstuffs - Detection of food allergens by molecular biological methods - Part 1: General considerations; English version of DIN EN 15634-1:2009-04

NF V03-052-1:2009 食品 使用分子生物学方法探测食品过敏原 第1部分 一般考虑

Foodstuffs - Detection of food allergens by molecular biological methods - Part 1 : general considerations.

BS EN 15634-1:2009 食品.使用分子生物学方法测定食物过敏原.一般考虑

This European Standard provides the overall framework for detection of sequences corresponding to species containing allergens using the polymerase chain reaction (PCR). It relates to the requirements for the specific amplification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified nucleic acid sequence. Guidelines, minimum requirements and performance criteria laid down in the European Standard are intended to ensure that comparable and reproducible results are obtained in different laboratories. This European Standard has been established for food matrices.

Foodstuffs - Detection of food allergens by molecular biological methods - Part 1: General considerations