This International Standard gives general guidelines for the enumeration of coliforms. It is applicable to — products intended for human consumption and for the feeding of animals, and — environmental samples in the area of food production and food handling, by means of the technique of counting colonies after incubation on a solid medium at 30℃ or at 37℃. NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30℃. This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.