13.080.30 土壤的生物特性 标准查询与下载

T/NAIA 011-2020 土壤脲酶活性的测定 苯酚钠-次氯酸钠比色法

本标准规定了土壤脲酶的测定方法，其中包括原理、试剂和溶液、仪器设备、分析步骤、 结 果计算及精密度。 本标准适用于各类土壤脲酶的测定。

Determination of Soil Urease Activity Sodium Phenate-Sodium Hypochlorite Colorimetric Method

T/NAIA 013-2020 土壤过氧化氢酶活性的测定 高锰酸钾滴定法

本标准规定了土壤过氧化氢酶的测定方法，其中包括原理、试剂和溶液、仪器设备、分 析 步骤、结果计算及精密度。 本标准适用于各类土壤过氧化氢酶的测定。

Determination of Soil Catalase Activity by Potassium Permanganate Titration

T/NAIA 010-2020 土壤蔗糖酶活性的测定 3,5-二硝基水杨酸比色法

本标准规定了土壤蔗糖酶的测定方法，其中包括原理、试剂和溶液、仪器设备、分析步 骤、 结果计算及精密度。 本标准适用于各类土壤蔗糖酶的测定。

Determination of Soil Sucrase Activity by 3,5-Dinitrosalicylic Acid Colorimetric Method

T/NAIA 009-2020 土壤微生物量氮的测定

本标准规定了土壤微生物量氮的测定方法。 本标准适用于各类土壤微生物量氮的测定。

Determination of soil microbial biomass nitrogen

BS ISO 16751:2020 土壤质量 非极性有机化合物的环境可用性 使用强吸附剂或络合剂测定潜在生物可利用部分和非生物可利用部分

What is ISO 16751 about?    ISO 16751 discusses soil quality. ISO 16751 specifies an extraction method to determine the bioavailable (potential and environmental available) fraction and the non-bioavailable fraction of a contaminant in soil using a “receiver phase” for an organic contaminant with strong sorbing or complexing properties.   The method is applicable for non-polar organic contaminants with an aqueous solubility of <100 mg/l. The method is applicable for soil and soil-like material including (dredged) sediments.   Who is ISO 16751 for?

Soil quality. Environmental availability of non-polar organic compounds. Determination of the potentially bioavailable fraction and the non-bioavailable fraction using a strong adsorbent or complexing agent

BS EN ISO 29200:2020 土壤质量 评估对高等植物的遗传毒性影响 蚕豆微核试验

1   Scope The purpose of this International Standard is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments. Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters, effluents, etc.

Soil quality. Assessment of genotoxic effects on higher plants. Vicia faba micronucleus test

BS EN ISO 17512-2:2020 土壤质量 用于确定土壤质量和化学品对行为影响的回避试验 弹跳虫试验（Folsomia candida）

1   Scope This part of ISO 17512 specifies a rapid screening method for evaluating the habitat function of soils based on the avoidance behaviour of springtails. The test is a rapid method that reflects the bioavailability of contaminants in natural soils and substances spiked into soils to Folsomia candida . In both cases, it is possible to establish a dose-response-relationship. The avoidance behaviour of the springtails is the measurement endpoint of the test. This test is not intended to replace the Collembola reproduction test.

Soil quality. Avoidance test for determining the quality of soils and effects of chemicals on behaviour - Test with collembolans (Folsomia candida)

BS EN ISO 21479:2020 土壤质量 确定污染物对土壤菌群的影响 植物叶脂肪酸组成评估土壤质量

1   Scope This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils. This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas. This method is applicable to: — soils from contaminated sites; — amended soils; — soils after remediation; — soil with waste products (e.g .  slurry, manure, sludge or composts). Alternatively, the quality of soils can be assessed by determining the Omega-3 index of Lactuca sativa seedlings grown in these soils under controlled conditions (i.e. phytotronic chamber) and by comparing these values to those obtained from control soils (see Annex B).

Soil quality. Determination of the effects of pollutants on soil flora. Leaf fatty acid composition of plants to assess soil quality

ISO 16751:2020 土壤质量.非极性有机化合物的环境有效性.用强吸附剂或络合剂测定潜在的生物可利用部分和非生物可利用部分

This document specifies an extraction method to determine the bioavailable (potential and environmental available) fraction and the non-bioavailable fraction of a contaminant in soil using a “receiver phase” for an organic contaminant with strong sorbing or complexing properties, for example, Tenax®1) or cyclodextrin, respectively. NOTE 1 The bioavailable fraction is defined in ISO 17402 as environmental bioavailability. The method is applicable for non-polar organic contaminants with an aqueous solubility of 3) and less for compounds with a higher solubility. The applicability is therefore defined for compounds with an aqueous solubility of

Soil quality — Environmental availability of non-polar organic compounds — Determination of the potentially bioavailable fraction and the non-bioavailable fraction using a strong adsorbent or complexi

ISO 23266:2020 土壤质量暴露于土壤中污染物的杀螨（Oppia nitens）繁殖抑制试验

This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intended to replace the earthworm or Collembola tests since it represents another taxonomic group (= mites; i.e., arachnids), nor the predatory mite test since this species represents a different trophic level and ecological niche. Effects of substances are assessed using standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil with similar properties to the soil sample to be tested (reference soil) or a standard soil (e.g., artificial soil). Information is provided on how to use this method for testing substances under temperate conditions. This document is not applicable to substances for which the air/soil partition coefficient is greater than 1, or to substances with vapour pressure exceeding 300 Pa at 25 °C. NOTE The stability of the test substance cannot be assured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.

Soil quality — Test for measuring the inhibition of reproduction in oribatid mites (Oppia nitens) exposed to contaminants in soil

BS EN ISO 21285:2020 土壤质量 土壤污染物对土壤螨（Hypoaspis aculeifer）繁殖的抑制

1   Scope This document specifies a chronic test method for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Hypoaspis aculeifer by – mainly – alimentary uptake. This method is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites under concern and waste materials (e.g. dredged material, municipal sludge from a wastewater treatment plant, composed material, or manure, especially those for possible land disposal). The reproduction (= number of juveniles) is the measured parameter of the test. The test reflects the bioavailability of a mixture of contaminants in natural soils (contaminated site soils) to a species which represents a trophic level which is not covered by other ISO standards. This test is not intended to replace the earthworm (see ISO 11268‑2) or Collembola (see ISO 11267) reproduction tests since this species belongs not only to a different trophic group but also a different taxonomic group (= mites; i.e. arachnids) than those used usually. Effects of substances are assessed using a standard soil , preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil . Depending on the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested ( reference soil ) or a standard soil (e.g. artificial soil). This document provides information on how...

Soil quality. Inhibition of reproduction of the soil mite (Hypoaspis aculeifer) by soil contaminants

BS EN ISO 21286:2020 土壤质量 DNA条形码鉴定生态毒理学试验物种

1   Scope This document specifies a protocol to identify ecotoxicological test specimens (mainly invertebrates and plants) to the species level, based on the DNA barcoding technique. This protocol can be used by laboratories performing DNA barcoding in order to standardize both the wet-lab and data analysis workflows as much as possible, and make them compliant with community standards and guidelines. This document does not intend to specify one particular strain for each test method, but to accurately document the species/strain which was used. NOTE 1 This does not imply that DNA barcoding is performed in parallel to each test run, but rather regularly (e.g. once a year, such as reference substance testing) and each time a new culture is started or new individuals are added to an ongoing culture. This document does not aim at duplicating or replacing morphological-based species identifications. On the contrary, DNA barcoding is proposed as a complementary identification tool where morphology is inconclusive, or to diagnose cryptic species, in order to ensure that the results obtained from different ecotoxicological laboratories are referring to the same species or strain. This document is applicable to identifications of immature forms which lack morphological diagnostic characters (eggs, larvae, juveniles), as well as the streamline identification of specimens collected in field monitoring studies, where large numbers of organisms from diverse taxa are classified. NOTE 2 In principle, all species regularly used in ecotoxicological testing can ...

Soil quality. Identification of ecotoxicological test species by DNA barcoding

BS EN ISO 11266:2020 土壤质量 有氧条件下土壤中有机化学品生物降解实验室测试指南

1   Scope This International Standard provides guidance on the selection and conduct of appropriate test methods for the determination of biodegradation of organic chemicals in aerobic soils. It does not describe any specific test method.

Soil quality. Guidance on laboratory testing for biodegradation of organic chemicals in soil under aerobic conditions

BS EN ISO 14239:2020 土壤质量 用于测量有氧条件下土壤中有机化学品矿化的实验室培养系统

1   Scope This document specifies six suitable incubation systems for measuring the rates and extent of mineralization of organic compounds in soil by measurement of carbon dioxide (CO 2 ) evolution. All incubation systems are applicable to soluble or insoluble compounds but choice of system depends on the overall purposes of the study. This document does not apply to the use of such systems for material balance studies, which are often test-substance specific.

Soil quality. Laboratory incubation systems for measuring the mineralization of organic chemicals in soil under aerobic conditions

BS EN ISO 15473:2020 土壤质量 厌氧条件下土壤中有机化学品生物降解实验室测试指南

1   Scope This International Standard gives guidance on the selection and method of appropriate tests for the determination of biodegradation of organic chemicals in soil samples under anaerobic conditions. NOTE If the method is intended for tests in the framework of the registration of chemicals, an OECD Guideline on soil degradation [20] gives useful guidance on additional test requirements.

Soil quality. Guidance on laboratory testing for biodegradation of organic chemicals in soil under anaerobic conditions

BS EN ISO 20130:2020 土壤质量 使用微孔板中的比色底物测量土壤样品中的酶活性模式

1   Scope This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β -galactosidase, α -glucosidase, β -glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.

Soil quality. Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates

EN ISO 20130:2020 土壤质量.用微孔板中的比色基质测量土壤样品中的酶活性模式

This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.

Soil quality - Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates (ISO 20130:2018)

EN ISO 29200:2020 土质.高等植物遗传毒性效应的评估.蚕豆(Vicia faba)微核试验

The purpose of ISO 29200:2013 is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments. Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters, effluents, etc.

Soil quality - Assessment of genotoxic effects on higher plants - Vicia faba micronucleus test (ISO 29200:2013)

EN ISO 11266:2020 土壤质量 好氧条件下土壤中有机化学品生物降解的实验室试验指南

Provides guidance on the selection and conduct of appropriate test methods for the determination of biodegradation of organic chemicals in aerobic soils. Does not describe any specific test method.

Soil quality - Guidance on laboratory testing for biodegradation of organic chemicals in soil under aerobic conditions (ISO 11266:1994)

EN ISO 15685:2020 土壤质量.潜在氮化合物和氮化合物的抑止物的测定.铵氧化物的快速试验

ISO 15685:2012 specifies a rapid method for the determination of the potential rate of ammonium oxidation and inhibition of nitrification in soils. This method is suitable for all soils containing a population of nitrifying microorganisms. It can be used as a rapid screening test for monitoring soil quality and quality of wastes, and is suitable for testing the effects of cultivation methods, chemical substances [except volatiles i.e. H > 1 (Henry's constant)], extracts of biosolids and pollution in soils.

Soil quality - Determination of potential nitrification and inhibition of nitrification - Rapid test by ammonium oxidation (ISO 15685:2012)