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DETERMNATION OF ALBUMIN CONCENTRATION IN URINE (DIAGNOSTICS OF MICROALBUMINURIA) |
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Determination of albumin is based on electrophoretic migration of its anionic form in the electric field and its direct detection by measuring the UV absorption in the 215–220-nm range. Prior to analysis sample aliquot was desalted either by ultra-diafiltration or on the “Sephadex® G-25” column.