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化学生物学实验技术>Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition
Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition
关键词: study polytopic membrane来源: 互联网
A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM™). SCAM™ is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. The strategy described can be adapted to any membrane system.
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