Manipulating the Cell Differentiation Through Lentiviral Vectors
The manipulation of cell differentiation is important to create new sources for the treatment of degenerative diseases or solve cell depletion after aggressive therapy against cancer. In this chapter, the use of a tissue-specific promoter lentiviral vector to obtain a myocardial pure lineage from murine embryonic stem cells (mES) is described in detail. Since the cardiac isoform of troponin I gene product is not expressed in skeletal or other muscle types, short mouse cardiac troponin proximal promoter is used to drive reporter genes. Cells are infected simultaneously with two lentiviral vectors, the first expressing EGFP to monitor the transduction efficiency, and the other expressing a puromycin resistance gene to select the specific cells of interest. This technical approach describes a method to obtain a pure cardiomyocyte population and can be applied to other lineages of interest.
- Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells
- Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking
- The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms
- The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids
- Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope
- In Silico Identification of Novel G Protein Coupled Receptors
- The Problems and Pitfalls of Radioligand Binding
- In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis
- Reliable Method for Detection of Programmed Cell Death in Yeast
- 人造血干细胞扩增的研究进展