MicroRNA Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing
The discovery of small regulatory RNA molecules during the last years has changed our understanding of many biological and pathological processes. The most prominent and best analyzed class of these small regulatory noncoding RNAs is composed of the microRNAs. The analysis of microRNA expression patterns is now widely used in biology and pathology employing a range of methodologies. However, procedures for data processing and calculations are far from standardized and differ considerably between published studies. This makes comparisons and meta-analyses still quite difficult. In this chapter, we describe a modified method for normalization and processing of raw data obtained if utilizing fluorescence-labeled bead technology from Luminex. Inc.
- E1A-Mediated Gene Therapy
- Single-Chain Fv-Based Affinity Purification of the Cellular Stress Protein gp96 for Vaccine Development
- Transforming Growth Factor Beta: A Plasma Tumor Marker
- Examination of CYP3A and P-Glycoprotein-Mediated DrugDrug Interactions Using Animal Models
- Introduction to the Analysis of the Human G-Banded Karyotype
- Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection
- Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma
- 用LIPO2000转染HepG2细胞的问题
- Flow Cytometric Monitoring of Fluorescent Drug Retention and Efflux
- p53-Induced Gene Expression Analysis