Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice
Th1 immunity protects against tuberculosis infection in mice and humans. The widely used BCG vaccine primes CD4 and CD8 T cells through signaling mechanisms from dendritic cells and macrophages. The latter express MHC-II and MHC-I molecules through which peptides from BCG vaccine are presented to CD4 and CD8 T cells, respectively. Since BCG sequesters within a phagosome that does not fuse with lysosomes, generation of peptides within antigen-presenting cells infected with BCG occurs with reduced efficiency. We demonstrate that activation of DCs containing BCG vaccine with rapamycin leads to an enhanced ability of DC vaccines to immunize mice against tuberculosis. Coadministration of rapamycin with BCG vaccine also enhanced Th1 immunity. We propose that rapamycin-mediated increase in Th1 responses offers novel models to study mTOR-mediated regulation of immunity.
- 粗肌丝(think filament)
- Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays
- Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma
- Identification and Isolation of Adult Liver Stem/Progenitor Cells
- Chemotaxis of Slow Migrating Mammalian Cells Analysed by Video Microscopy
- 几种细胞培养基M199中维生素含量的测定方法
- Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry
- In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears
- Sperm Selection Based on Electrostatic Charge
- 不同细胞siRNA转染效率检测讨论