In Vitro Assays for Neural Stem Cell Differentiation
Neural stem cells (NSCs) are an undifferentiated population of cells residing in the tissue lining the ventricular system of both the embryonic and adult mammalian central nervous system (CNS) (1 –3 ). When isolated under the appropriate genetic or epigenetic conditions, NSCs have the ability to self renew and produce clinical amounts of cells that may be well characterized in vitro and in vivo, cryopreserved, or ready for therapeutic use. Upon transplantation into the adult CNS, NSCs integrate seamlessly within the host tissue and differentiate according to the local microenvironment into any one of the principal CNS cell types (neurons, astrocytes, or oligodendrocytes). These attributes make NSCs a good source of cells for cell replacement therapy for neurological disorders. However, this source of neural tissue will not reach the clinical stage unless it is well characterized both in vitro and in animal models.
- Exo utero Surgery
- Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members
- Detection of Nuclear -catenin in Xenopus Embryos
- Inhibition of DNA Synthesis by Ionizing Radiation
- Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic
- Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishment After Myocardial Injury
- Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies
- 人类若干性状的遗传特性及其调查分析
- 对控基因(pair-rule genes)
- 显微镜放射自显影样品的制备及观察