Enzyme Analysis: Cathepsin D as an Example
In enzyme analysis, capillary electrophoresis (CE) offers the ease of product separation from the substrate, with the ability to use expensive reagents in microvolumes. In CE, enzymes can be measured either as mass (when they are present in high concentration) by direct light absorbency, or by catalytic activity. For example, the protease enzyme, savinase, which is used as an ingredient in washing powder, was determined directly by its absorbency at 200 nm (1 ). For catalytic activity measurements, the substrate, the product, or both can be measured in CE without the need for coupling reactions. Because of the increased sensitivity, most CE methods measure enzymes by their catalytic activity on a substrate. To accomplish this by CE, several approaches have been used.
- Expression and Purification of Membrane-Type MMPs
- Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer
- Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts
- Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure
- Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
- Transfection of Osteoclasts and Osteoclast Precursors
- 结晶紫染色测定细胞数
- Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
- DNA Laddering
- 载体