Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells

Humana Press| 1588290328 | Edition - 2002-12-06 | PDF | 546 pages| 3.9 MB

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

Table of Contents

Part I. Mammalian Cell Culture

Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture

Nigel Jenkins

Genetic Approaches to Recombinant Protein Production in Mammalian Cells

Peter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, and Hansjörg Hauser

Protein Expression Using Transgenic Animals

William H. Velander and Kevin E. van Cott

Mammalian Cell Culture: Process Development Considerations

Steven Rose, Thomas Black, and Divakar Ramakrishnan

Part II. Microbial Cell Culture

Natural Products: Discovery and Screening

Matthew D. Hilton

Genetic Engineering Solutions for Natural Products in Actinomycetes

Richard H. Baltz

Culture Medium Optimization and Scale-Up for Microbial Fermentations

Neal C. Connors

Part III. Plant Cell Culture

Functional Genomics for Plant Trait Discovery

Sam Reddy, Ignacio M. Larrinua, Max O. Ruegger, Vipula K. Shukla, and Yuejin Sun

Molecular Tools for Engineering Plant Cells

Donald J. Merlo

Plant Cell Culture: A Critical Tool for Agricultural Biotechnology

Joseph F. Petolino, Jean L. Roberts, and Ponsamuel Jayakumar

Expression of Recombinant Proteins via the Plastid Genome

Jeffrey M. Staub

Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds

Maurice M. Moloney

Part IV. Critical Tools for Biotechnology

Genome Sequencing and Genomic Techniques in Drug Discovery and Development

Lawrence M. Gelbert

Proteomics

Gerald W. Becker, Michael D. Knierman, Pavel Shiyanov, and John E. Hale

Metabolic Flux Analysis, Modeling, and Engineering Solutions

Walter M. van Gulik, Wouter A. van Winden, and Joseph J. Heijnen

Advances in Analytical Chemistry for Biotechnology: Mass Spectrometry of Peptides, Proteins, and Glycoproteins

Jeffrey S. Patrick

DNA Shuffling for Whole Cell Engineering

Steve del Cardayre and Keith Powell

Cell Culture Preservation and Storage for Industrial Bioprocesses

James R. Moldenhauer

Index