Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients
Results obtained from preclinical studies have shown that endothelial progenitor cells (EPCs) play a crucial role in tumor growth and metastasis. In the clinic, EPCs are present in the peripheral blood of cancer patients in higher numbers than in healthy subjects. These cells are mobilized from the bone marrow compartment to the periphery in response to certain cytokines and growth factors. Growing body of evidence suggests that following acute cytotoxic drug therapy levels of circulating EPCs (CEPs) can change significantly in both mouse and human. These changes may predict the efficacy of some anticancer drug treatments. Therefore, the validation and standardization of a procedure to detect CEPs and monitor their kinetic is an important step towards the use of such cells as a possible biomarker to predict clinical outcome. In this chapter, we describe a flow cytometry technique to detect CEPs obtained from human blood specimens stored in both fresh and frozen conditions.
- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections
- Autogene Selections
- RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs
- Human Peripheral Blood Mononuclear Cell Preparation
- Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
- 细胞培养实验的经验总结
- Evaluation of Rapamycin-Induced Cell Death
- 细胞增殖周期
- 双链断裂重组模型(model of double-strand breaks recombination)
- 细胞计数及活力测定方法