Gene Expression Profiling of Mouse Epidermal Keratinocytes
DNA microarray technology is a powerful tool for genome-wide gene expression analysis of biological samples including skin and epidermal keratinocytes. Here we review the methodology for expression profiling analysis of skin tissue or purified keratinocytes from mice. We explained the methodology and protocols for RNA preservation and purification, RNA quality and integrity tests, and DNA microarray technology types that can be used. Furthermore, using a data set of mice samples, we explained how to perform chip raw data preprocessing and normalization, differential expression analysis, gene-clustering and functional analysis of gene deregulation.
- Basics of Flow Cytometry
- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media
- Culture of Adipose Tissue and Isolated Adipocytes
- Control of the Interferon Response in RNAi Experiments
- Real-Time BRET Assays to Measure G Protein/Effector Interactions
- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization
- Gene Expression in Mouse Oocytes by RNA-Seq
- Interspecific Chimeras: Transplantation of Neural Crest Between Mouse and Chick Embryos
- Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells
- 受体( receptor)