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前沿科技及其它>Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions
Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions
关键词: yeast mammalian two-hybrid来源: 互联网
An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.
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