挑蚀斑的方法
关键词: 蚀斑来源: 互联网
拿微量移液器,安黄枪头的,100μl或200μl的均可。
等蚀斑形成后,先在板子背面画好圈,标记好斑的大小。倒扣板子,拿起来,安上枪头,用灭菌的小剪子根据斑大小剪掉枪尖,在火上迅速过一下,对着画好的斑,先扎再吸,胶就起来了。然后再安个枪头,再板上再吸一下,就好了。挑的斑可放在离心管,冻存管都行,加1ml的DMEM,如果需要血清,浓度在2%就可以。
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