Construction and Isolation of Recombinant MVA
Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its clear safety record, and it can be handled under biosafety level 1 conditions. Despite its replication deficiency in human and most mammalian cells, MVA provides high-level gene expression and has proven to be immunogenic when delivering heterologous antigens in animals and humans. This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.
- 发酵过程控制与优化
- 生物档案:微生物(第二部分)
- Construction and Isolation of Recombinant MVA
- HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
- The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Stud
- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease
- Analysis of Chromatin Organization by Deep Sequencing Technologies
- Genetic Analysis of Food Spoilage Yeasts
- Chancroid