Biolog Phenotype Microarrays
Phenotype microarrays nicely complement traditional genomic, transcriptomic, and proteomic analysis by offering opportunities for researchers to ground microbial systems analysis and modeling in a broad yet quantitative assessment of the organism’s physiological response to different metabolites and environments. Biolog phenotype assays achieve this by coupling tetrazolium dyes with minimally defined nutrients to measure the impact of hundreds of carbon, nitrogen, phosphorous, and sulfur sources on redox reactions that result from compound-induced effects on the electron transport chain. Over the years, we have used Biolog’s reproducible and highly sensitive assays to distinguish closely related bacterial isolates, to understand their metabolic differences, and to model their metabolic behavior using flux balance analysis. This chapter describes Biolog phenotype microarray system components, reagents, and methods, particularly as they apply to bacterial identification, characterization, and metabolic analysis.
- 几种常见的微生物基础实验
- 标准菌株质控品
- 酵母固定化细胞的肌动蛋白染色法
- Construction of Mouse Adenovirus Type 1 Mutants
- Mixed Infections with Rotaviruses: Protocols for Reassortment, Complementation, and Other Assays
- NMR-Based Isotopologue Profiling of Microbial Carotenoids
- 猴B病毒(HS)酶联免疫分析(ELISA)
- An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods
- Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis
- Antibiotic Resistance Profiling