Reagents:

ChIP sonication Buffer (1% Triton X-100,0.1% Deoxycholate,50 mM Tris 8.1,150 mM NaCl,5 mM EDTA):

10 ml 10% Triton X-100

1 ml 10% Deoxycholate

5 ml 1 M Tris-Cl pH 8.1

1 ml 0.5 M EDTA

3 ml 5 M NaCl

80 ml Water

Just before use,add 10μl Aprotinin,10μl Leupeptin,and 5μl PMSF to each 10 ml.

High Salt Wash Buffer (1% Triton X-100,0.1% Deoxycholate,50 mM Tris-8.1,500 mM NaCl,5 mM EDTA)

10 ml 10% Triton X-100

1 ml 10% Deoxycholate

5 ml 1M Tris-8.1

1 ml 0.5M EDTA

10 ml 5M NaCl

73 ml Water

(LiCl Immune Complex Wash Buffer)

25 ml 1mliCl

5 ml 10% IGEPAL

5 ml 10% Deoxycholate

1 ml 1M Tris-8.1

200μl 0.5M EDTA

64 ml Water

Protease inhibitors (add 10μl of each to 10 ml of PBS or sonication buffer)

Leupeptin 2 mg/ml in water

Aprotinin 2 mg/ml in water

PMSF 0.2 M

5 M NaCl

1X TE Buffer (10mM Tris,8.1,1 mM EDTA)

0.5 M EDTA

1 M Tris-Cl,pH 6.8

Protein A/G Agarose (Santa Cruz #sc-2003)

Proteinase K (19 mg/ml,Boehringer Mannheim # 1964372)

10X proteinase K buffer

Elution Buffer (1% SDS,0.1 M NaHCO3,0.01 mg/ml Herring sperm DNA)

10 mg/ml Herring Sperm DNA

37% Formaldehyde (ACS reagent grade)

1.25 M glycine