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Hybridization for Spectral Karyotyping (SKY)
关键词： Hybridization Spectral Karyotyping SKY 光谱核型分析技术 杂交来源： 互联网
Reagents
dH 2O Ethanol, absolute (70%, 90%, and 100%) Formamide, deionized Ambion, Cat. No. 9342 HCL, 1N Rubber Cement SSC, 20X
Preparation
70% Formamide/2X SSC 20X SSC 10 ml dH 2O 20 ml deionized formamide 70 ml Adjust to pH 7.0 with 1N HCL Aliquot and store at –20°C. Pre-cool 70% ethanol to 0°C
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