Linking Emulsion PCR Haplotype Analysis
The experimental measurement of haplotype requires the determination of two or more genotypes on the same DNA molecule. Because such measurements are much more complicated than measurements of genotypes, haplotypes are typically inferred using population data for linkage disequilibrium between the markers of interest. We have developed a method for molecular haplotyping, linking emulsion PCR (LE-PCR), and have demonstrated that the method is sufficiently robust to determine haplotypes for multiple markers in a population setting. LE-PCR uses emulsion PCR to isolate single template molecules for simultaneous PCR of widely spaced markers and uses linking PCR to fuse these amplicons into one short amplicon, which maintains the phase of the markers. LE-PCR is illustrated for polymorphisms in human paraoxonase 1 (PON1 ) that have been shown to affect transcriptional activity and substrate specificity in the detoxification of organophosphates.
- Efficient Retroviral Gene Transfer to Epidermal Stem Cells
- Comparative Genomic Hybridization: Microarray Design and Data Interpretation
- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Com
- Bioluminescence Resonance Energy Transfer: An Emerging Tool for the Detection of ProteinProtein Interaction in Living Cells
- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs
- Aggregation of Embryos and Embryonic Stem Cells
- In Vitro DNA Recombination by Random Priming
- Visualization of DNA Topoisomerases by Electron Microscopy
- Using Recombineering to Generate Point Mutations: The Oligonucleotide-Based Hit and Fix Method
- Establishment of the DNA Repair-Defective Mutants in DT40 Cells