End-Rescue of YAC Clone Inserts by Inverse PCR
关键词: end-rescue yac clone inserts inverse pcr来源: 互联网
The termini of yeast artificial chromosome (YAC) inserts can serve as probes or sequence tagged sites (STSs) that are useful in genomic analysis. For example, these elements can be used to:
1. | Build physical maps and YAC contigs; |
2. | Characterize and orient YAC clones; |
3. | Assess chromosomal origin and YAC chimerism; and |
4. | Construct conventional and rare-cutting restriction maps. |
推荐方法
- Asynchronous PCR
- Staden: Searching for Restriction Sites
- Array CGH in Brain Tumors
- Mucosal Immunization with DNA Vaccines
- Surface Plasmon Resonance Imaging on Polypyrrole Protein Chips: Application to Streptavidin Immobilization and Immunodetection
- Inducible microRNA-Mediated Knockdown of the Endogenous Human Lamin A/C Gene
- Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis
- Rat Genomics Applied to Psychiatric Research
- Radiolabeling for Two-Dimensional Gel Analysis
- Efficient Viral Gene Transfer to Rodent Hearts In Vivo