热门搜索词：
Functional Analysis of Exosomal MicroRNA in CellCell Communication Research
Circulating microRNAs (miRNAs), also known as secretory miRNAs, are packaged in small membrane vesicles called exosomes. These exosomal miRNAs are secreted from various cell types and incorporated inside the recipient cells. The functions of exosomal miRNAs are poorly understood, but some reports have shown their essential roles in cancer development. Therefore, methods to study the function of exosomal miRNAs not only in vitro but also in vivo might be essential. We have analyzed the function of exosomal miRNAs by miRNA-enriched exosomes both in vitro and in vivo. In this chapter, the methods to concentrate the targeted miRNAs are provided. This simple and useful method enables the study of the precise mechanisms of exosomal miRNAs under physiological and pathological conditions.
- Dual DNA/RNA Extraction
- Use of Site-Specific ProteinDNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcr
- Calcium Phosphate Mediated Gene Transfer into Established Cell Lines
- Using Mini-genes to Identify Factors That Modulate Alternative Splicing
- Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes
- Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae
- Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler
- In Silico Protein Motif Discovery and Structural Analysis
- Establishment of Embryonic Stem Cells from Rat Blastocysts
- The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane
移动版：