Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikC实验方法详情页

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Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikC

关键词： two-stage two stage polymerase chain reaction protocol allowing introduction multiple mutations deletions insertions using quikc来源：互联网

The invention of polymerase chain reaction (PCR) (1,2) provided a powerful tool to modify DNA sequences in genetic engineering. With numerous mutagenesis methods available, such as traditional sequential PCR (3), “megaprimer PCR”(4–7), marker-coupled PCR (8), and so on, introducing changes to DNA sequences has become less tedious and more efficient. Recently, marketed site-directed mutagenesis (SDM) kits, such as Transformer™ Site-Directed Mutagenesis Kit (Clontech, San Francisco, CA), and Altered Site� II in vitro Site-Directed Mutagenesis Systems (Promega, Madison, WI), even eliminate the necessity of subcloning the amplified fragment.

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