Exonuclease III Footprinting on Immobilized DNA Templates
DNA footprinting is a widely used method to locate the binding sites of protein on the DNA. It is based on the observation that a protein bound to DNA protects it from degradation by an enzyme or chemical reagent. Exonuclease III is a suitable probe to analyze the boundaries of a protein when it is necessary to eliminate any excess unbound DNA from the reaction to avoid background problems. In combination with biotin-labeled DNA that is bound to streptavidin-coated magnetic particles, information on the precise position of a DNA bound protein is available within a few hours. The position of the archaeal RNA polymerase at different stages of transcription in the Pyrococcus furiosus in vitro transcription system was analyzed by this method.
- Antibody-Directed Enzyme-Prodrug Therapy
- C. elegans Sirtuins
- Lentivirus-Mediated Gene Expression
- Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer
- Enzyme-Free Cloning of PCR Products and Fusion Protein Expression
- Detecting MicroRNA in Human Cancer Tissues with Fluorescence In Situ Hybridization
- LNA-FISH for Detection of MicroRNAs in Frozen Sections
- An In Vitro Transcription System from BmN Cells of the Silkworm, Bombyx mori
- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains
- Application of PCR to Transgenic Plants