In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
Base excision repair (BER) is an important mechanism to maintain genomic stability. Here we offer a set of protocols to quantitatively analyze BER capacity in whole cell-free yeast extracts. Cell-free yeast extracts were obtained by a French press procedure and repair capacities were measured by using oligonucleotide substrates. Repair products were separated by polyacrylamide gel electrophoresis and detected by autoradiography. These set of methods allow the analysis of different kinds of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
- Chromatin Immunoprecipitation Using Microarrays
- Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy
- Backcross Populations and Near Isogenic Lines
- Yeast Artificial-Chromosome (YAC) Cloning Systems
- miRNAs in Human Cancer
- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs: II. Automatic Injection
- The Integron/Gene Cassette System: An Active Player in Bacterial Adaptation
- Master Regulators of Posttranscriptional Gene Expression Are Subject to Regulation
- Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review
- Supported Lipid Bilayers and DNA Curtains for High-Throughput Single-Molecule Studies