The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues
Research in molecular genetic studies and in transgenic biotechnology has resulted in excellent advancements in exploiting gene transfer techniques. Basic research in transcriptional and/or translational control, protein engineering, cellular immunological mechanisms, and developmental genetic processes using transient or stable transgenic approaches have generated the main body of information in our current understanding of various molecular biology and cell biology disciplines. Parallel with this development, the gene transfer and transgene expression techniques have also been extremely useful in their application to plant and animal (including mammalian) biotechnologies. Specifically, analyses of promoter activity and usage have played important roles in generation of genetically engineered plants, transgenic animals, and recombinant or transgenic protein products in various microbial fermenters or plants or in mammalian cell bioreactors. More recently, various gene transfer and promoter usage technologies have also been evaluated extensively for application to gene therapy research.
- Adenovirus-Mediated Gene Transfer
- Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors
- Measuring Changes in Chromatin Using Micrococcal Nuclease
- Family-Based Linkage Disequilibrium Tests Using General Pedigrees
- Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry
- Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer
- Capillary Electrophoresis of an 11-Plex mtDNA Coding Region SNP Single Base Extension Assay for Discrimination of the Most Commo
- Ethylation Interference
- Bioinformatics Analysis of Microarray Data
- Derivation, Culture, and In vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts