Neural Cell Culture Techniques
Cell culture has proven to be a very powerful approach in addressing neurobiological questions. The reasons for this include the ability to isolate the effects of specific variables on cells and, more importantly, to ask questions of a specific cell type in isolation of other cells. The power of cell culture is also its weakness since the nervous system does not consist of cells working in isolation, but rather it consists of communities of cells that interact. Because of this, one must use considerable caution in interpreting data obtained from cultured cells (Juurlink and Hertz, 1985); however, one must also remember the words of Margaret Murray, one of the pioneers of neural cell culture, that “anything that a cell is seen to do in culture must be counted among its potentialities” (Murray, 1977).
- Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels
- NeuropeptideYY1 Receptor-Deficient Mice: Generation and Characterization
- The Mouse Defense Test Battery: A Model Measuring Different Facets of Anxiety-Related Behaviors
- Subcellular Fractionation of Brain Tissue Using Free-Flow Electrophoresis
- Strategies for Regulating Calpain Activities in Living Cells
- Astrocytes
- Telemetry in Mice: Applications in Studies of Stress and Anxiety Disorders
- Alcohol Tolerance: Methodological and Experimental Issues
- Interneuron Loss as a Cause of Seizures: Lessons from Interneuron-Deficient Mice
- Denaturing High-Performance Liquid Chromatography and Sequence Analyses for MECP2 Mutations in Rett Syndrome