A Method for Analyzing ProteinProtein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Trans
For more than a decade, fluorescence resonance energy transfer (FRET) imaging methods have been developed to study dynamic interactions between molecules at the nanometer scale in live cells. Here, we describe a protocol to measure FRET by the acceptor-sensitized emission method as detected by total internal reflection fluorescence (TIRF) imaging to study the interaction of appropriately labeled plasma membrane-associated molecules that regulate the earliest stages of antigen-mediated signaling in live B lymphocytes. This protocol can be adapted and applied to many cell types where there is an interest in understanding signal transduction mechanisms in live cells.
- USE OF THE LIGHT MICROSCOPE
- Lymphoblastoid Cells Exposed to Low-Frequency Magnetic Fields: Study by Atomic Force Microscopy
- Microarray Image Scanning
- Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)
- Fluorescence Methods to Study DNA Translocation and Unwinding Kinetics by Nucleic Acid Motors
- PET and PET-CT of Lung Cancer
- Correlative Microscopy of Individual Cells: Sequential Application of Microscopic Systems with Increasing Resolution to Study th
- Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA Bead Assays and Flow Cytometry with 2100 Bioanalyzer
- Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue
- Light Sheet Microscopy in Cell Biology