Transposon Tagging with AC/Ds in Arabidopsis
Transposon tagging provides an effective method of isolating plant genes by identifying a mutation tagged by a DNA insertion, and then using the DNA tag to isolate the mutant gene (reviewed m ref. I ). The first examples of this approach in plants involved transposon tagging in maize and Antirrhinum using endogenous transposons. More recently, maize transposons were shown to be active in a wide range of plant species, and in some of these have facilitated the use of transposon tagging (2 ‐5 ). In Arabidopsis, Actzvator/Dissoclation (AC/ Ds) have been used to isolate recessive loss-of-function mutations (6 —8 ), dommant gain of function mutations (9 ) and to isolate genes based on then patterns of expression (10 ,11 ).
- 质壁分离与质壁分离复原及K+、Ga²+对质壁分离的影响
- 波斯红草
- 苦木科(Simaroubaceae)
- Regeneration in Arabidopsis Tissue Culture
- In Vitro Conservation and Cryopreservation of Ornamental Plants
- 姜(Zingiber offieinale)
- Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons
- 第四纪冰川
- Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
- 棕榈(Trachycarpus fortunei)