Genetic Screens to Identify Plant Stress Genes
A powerful means to learn about gene functions in a developmental or physiological context in an organism is to isolate the corresponding mutants with altered phenotypes. Diverse mutagenic agents, including chemical and biological, have been widely employed, and each comes with its own advantages and inconveniences. For Arabidopsis thaliana , whose genome sequence is publicly available, the reliance of reverse genetics to understand the relevant roles of genes particularly those coding for proteins in growth and development is now a common practice. Identifying multiple alleles at each locus is important because they can potentially reveal epistatic relationship in a signaling pathway or components belonging to a common signaling complex by their synergistic or even allele-specific enhancement of the phenotypic severity. In this article, we describe mutagenesis by using ethyl methanesulfonate (EMS) and transfer (T)-DNA-mediated insertion or activation tagging as applied to the most widely used genetic plant model A. thaliana . Also, we demonstrate the utility of several genetic screening approaches to dissect adaptive responses to various abiotic stresses.
- 叶绿体色素的分离
- 植物组织培养技术简介
- 双子叶植物纲
- 舌状花
- Determination of ADP-Ribosyl Cyclase Activity, Cyclic ADP-Ribose, and Nicotinic Acid Adenine Dinucleotide Phosphate in Tissue Ex
- Syntheses of Tetrapyrroles
- 薄荷
- Measurements of Viability Suitable for Plant Tissue Cultures
- Protein Tagging for Chromatin Immunoprecipitation from Arabidopsis
- In Situ Analysis of DNA Methylation in Plants