DNA extraction for Buccal Swabs实验方法详情页 - 分析测试百科网

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DNA extraction for Buccal Swabs

关键词： dna extraction来源：互联网

实验步骤

1. Collect buccal cells on swab and allow the swab to dry. Drying time is approximately 10 to 15 minutes.

Note: Due to the low volume of solution used for DNA extraction, a foam tipped swab should be used. Swabs with fibrous tips, such as cotton or dacron, should be avoided because the solution can not be recovered efficiently.

2. Pipette 200 mL of Extraction Solution into a 1.5 ml microcentrifuge tube. Add 25 mL of Tissue Preparation Solution to the tube and pipette up and down to mix.

Note: If several extractions will be performed, sufficient volumes of Extraction and Tissue Preparation Solutions may be pre-mixed in a ratio of 8:1 up to 2 hours before use.

3. Place dried buccal swab into the solution and incubate at room temperature for 1 minute.

4. Twirl swab in solution 10 times and then remove excess solution from the swab into the tube by twirling swab firmly against the side of the tube. Discard the swab. Close the tube and vortex briefly.

5. Incubate sample at room temperature for 10 minutes.

6. Incubate sample at 95 °C for 3 minutes.

7. Add 200 mL of Neutralization Solution B to sample and mix by vortexing.

8. Store the neutralized extract at 4 °C or use immediately in PCR. Continue with Section C, step 1.

Note: Extracts may be stored at 4 °C for at least 6 months without notable loss in most cases.

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