Visualisation of Lymphoid Organ Development
This chapter provides information on imaging tools that can be employed to visualise and study lymphoid organ development. We focus on the use of genetically modified mouse models that take advantage of fluorescent protein expression in discrete cell populations, thus allowing live cell imaging during lymphoid organogenesis. We discuss approaches that allow characterisation of the cell types involved in the formation of lymphoid organs, including (i) functional assays in explant organ cultures and (ii) high-resolution whole-mount immunostaining methods, which are useful for the characterisation of specific cell populations in the context of the whole developing organ.
- EGFR单抗与肿瘤治疗进展
- 猪瘟单克隆抗体ELISA
- 关于ELISPOT试验技术中对细胞的处理问题
- 免疫荧光技术的实验方法及其分类
- Tracking Antigen-Specific Lymphocytes In Vivo
- Tolerogenic Dendritic Cells and Induction of T Suppressor Cells in Transplant Recipients
- Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium
- Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor
- Genetic Engineering of Dendritic Cells Using Retrovirus-Based Gene Transfer Techniques