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抗体抗原实验>Use of cDNA Library Expression Cloning to Identify Components of Heterodimeric Receptor Complexes
Use of cDNA Library Expression Cloning to Identify Components of Heterodimeric Receptor Complexes
关键词: expression cloning来源: 互联网
Expression cloning in mammalian host cells has revolutionized immunology. The current protocol was originally developed by Dr. Brian Seed in 1987 (1 ), and it has gone through a number of improvements over the years. The most important feature of this technique has been to exploit the exquisite specificity of antibodies for antigens by developing a method permitting gene cloning in a mammalian cell system.
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