实验方法> 生物化学技术> 化学生物学实验技术>Microwave Paraffin Techniques for Botanical Tissues

Microwave Paraffin Techniques for Botanical Tissues

关键词: microwave paraffin techniques来源: 互联网

The popularity of in situ hybridization (ISH) of nucleic acids and immunolocalization of proteins has caused a resurgence of interest in paraffin microtechnique by the plant biology community. However, the amount of time required for proper anatomical preservation of plant tissues results in degradation of nucleic acids and proteins in the sample (Jackson, 1991). There are protocols that may be used to reduce degradation, but they often result in poor anatomical preservation and target (nucleic acid, protein) conservation (Jackson, 1991; Kouchi and Hata, 1993 ; Ruzin, 1999 ).

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