报告基因检测的精明之选—LipoD293DNA转染试剂
使用lipoD293转染试剂来转染哺乳动物细胞（比如Hela 、PC3），以期检测荧光酶报告基因，同其他转染试剂相比较，您会得到更多的荧光酶读数，更少的细胞死亡率。
使用unsupplemented RPM11640/DMEM替代Opti-MEM培养基来稀释lipoD293及DNA（luciferase reporters），您会得到更高的荧光酶读数（提高一倍），并且能节省更多的经费。
如下是实验过程中的几个实验要点：
1、使用相同类型的培养基（but unsupplemented）来稀释lipoD293试剂及luciferase reporterDNA（例如，若是细胞是用RPM11640-based培养基培养的，那么可选用unsupplemented RPM11640作为稀释溶液）；
2、转染前及转染后，没有必要更换培养基；
3、将稀释的lipoD293试剂加入稀释的DNA，混匀，在室温下孵育15-20分钟。
Smart choice for reporter assay with LipoD293 DNA transfection reagent.
Use lipoD293 DNA transfection reagent for transfection of multiple mammalian cell lines (such as Hela and PC3) with luciferase reporters, you will get much higher luciferase readings than other transfected reagents used on our lab for luciferase reading, but observe little cell death in transfected cells in our condition!
Use unsupplemented RPMI1640 / DMEM instead of Opti-MEM medium to dilute both lipoD293 and DNA (luciferase reporters), you will get further higher luciferase readings (one-fold increase) and save more money !
Several key points in the protocol:
1) Use the same type of culture medium (but unsupplemented) for diluting lipoD293 reagent and luciferase reporter DNA( for example, if the cells are cultured in RPMI1640-based medium, then use unsupplemented RPMI1640 as dilution medium);
3) No need to change medium either before or after transfection;
4) Add diluted lipoD293 reagent to diluted DNA and then incubate the mixture at room temperature for 15~20 minutes;
5) Make lysates for luciferase assay 16~48hrs post transfection
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