Foodstuffs - Determination of zearalenone and trichothecenes including deoxynivalenol (DON) and its acetylated derivatives (3-acetyl- DON and 15-acetyl-DON)@ nivalenol (NIV) and T-2 and HT-2 toxin in cereals and cereal products by LC-MS/MS
This document describes a procedure for the determination of nivalenol (NIV)@ deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON)@ HT-2 and T-2 toxins (HT-2@ T-2) and zearalenone (ZEA) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) after cleanup by solid phase extraction (SPE). The method has been validated with both contaminated and spiked samples of wheat@ wheat flour@ and wheat crackers. Validation levels for NIV ranged from 27@7??/kg to 377@8 ??/kg. Validation levels for DON ranged from 233@9??/kg to 2420@0 ??/kg. Validation levels for 3-acetyl-DON ranged from 18@5??/kg to 136@5 ??/kg. Validation levels for 15-acetyl-DON ranged from 11@4??/kg to 141@8 ??/kg. Validation levels for HT-2 ranged from 6@6 ??/kg to 133@8 ??/kg. Validation levels for T-2 ranged from 2@1 ??/kg to 37@6 ??/kg. Validation levels for ZEA ranged from 31@6??/kg to 229@7 ??/kg Laboratory experiences have shown that this method is also applicable to barley and oat flour@ and rye based crackers [5]@ however@ this has not been validated in a collaborative study.