This International Standard specifies a method for the determination of the contents of free α-, β-, γ- and δ-
tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).
For products containing tocopherol or tocotrienol esters, it is necessary to prepare the unsaponifiable matter.
NOTE A suitable method involving a cold saponification procedure is described in annex A for information only.