BS ISO 14674-2005 奶和奶粉.黄曲霉毒素M1含量的测定.免疫亲和色谱法清洁和薄层色谱法测定
Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by thin-layer chromatography
This International Standard specifies a method for the determination of the aflatoxin M1 (AFM1) content of milk and milk powder by a method including a clean-up step using immunoaffinity chromatography followed by a thin-layer chromatography (IAC-TLC). The method is applicable to raw milk, low fat or skimmed liquid milk and milk powder. The lowest quantity of AFM1 that can commonly be determined is 2 ng, which corresponds to a limit of quantification close to 0,10 μg/l for liquid milk or dissolved milk powder (for a spot of 20 μl).