This International Standard gives general guidelines for the enumeration of coliforms. It is applicable to. products intended for human consumption and for the feeding of animals, and. environmental samples in the area of food production and food handling,by means of the technique of counting colonies after incubation on a solid medium at 30 °C or at 37 °C.NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30 °C.This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.