ASTM E2317-04(2012)
用海洋Meiobenthic桡足类甲壳动物进行复原的基于微板的寿命周期毒性试验的标准指南

Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod

标准号

ASTM E2317-04(2012)

发布

2004年

发布单位

美国材料与试验协会

当前最新

ASTM E2317-04(2012)

 

 

引用标准

ASTM E1023 ASTM E1191 ASTM E1192 ASTM E1218 ASTM E1847 ASTM E380 ASTM E729 ASTM E943

适用范围

5. Significance and UseTop Bottom

5.1 Protection of a species requires prevention of unacceptable effects on the number, health, and uses of individuals of that species. A life-cycle toxicity test is conducted to determine changes in the numbers of individuals and offspring of a test species resulting from effects of the test material on survival, growth, gender ratios, endocrine function, genetic expression, fertility and reproduction (1-3).³ Information might also be obtained on effects of the material on the health (4) and uses of the species.

5.2 Published information about the sensitivities of several meiobenthic copepods to several common metals and organic toxicants have been reviewed (5). For most compounds tested/published to date, A. tenuiremis is acutely less sensitive than mysid and penaeid shrimp, similarly sensitive as amphipods, and often more sensitive than cladocerans (daphniids, specifically). Reference 96-h aqueous toxicity tests with cadmium at 30 g/kg salinity showed an LC50 for A. tenuiremis adults of 213 to 234 ??g/L (Chandler, unpub.). Reference toxicant tests with sodium dodecyl sulfate showed a 96-h LC50 of 13.3 to 15.5 mg/L (Chandler,unpubl.). A. tenuiremis is a comparatively new toxicity test organism, and an extensive database of species sensitivity to multiple aqueous test compounds is not yet available. Relative to other harpacticoid copepod studies in the literature, A. tenuiremis is more chronically sensitive than all other species published to date where there is comparative data (5).

5.3 Results of life-cycle tests with A. tenuiremis can be used to predict long-term effects at the individual and population levels likely to occur on copepods in field situations as a result of exposure under comparable conditions (1,2).

5.4 Results of life-cycle tests with A. tenuiremis might be used to compare the chronic sensitivities of different species and the chronic toxicities of different materials, and also study the effects of various environmental factors such as temperature, pH, and ultraviolet light on results of such tests.

5.5 Results of life-cycle......

ASTM E2317-04(2012)相似标准

推荐