ASTM E1295-01(2013)
用网纹水蚤 (Dubia) 进行三卵复原毒性试验的标准指南

Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia

被代替

标准号

ASTM E1295-01(2013)

发布

2001年

发布单位

美国材料与试验协会

替代标准

ASTM E1295-22

当前最新

ASTM E1295-22

 

 

引用标准

ASTM D1193 ASTM D3978 ASTM E1023 ASTM E1192 ASTM E1193 ASTM E1383 ASTM E1706 ASTM E729 ASTM E943 IEEE/ASTM SI 10

适用范围

5.1 Ceriodaphnia  was first used as a toxicity test organism by Mount and Norberg (4). Introduced for use in effluent and ambient water evaluations, Ceriodaphnia have also been a valuable addition to single chemical test procedures.

5.2 Protection of a population requires prevention of unacceptable effects on the number, weight, health, and uses of the individuals of that species, or species for which the test species serves as a surrogate. A three-brood toxicity test is conducted to help determine changes in survival and the number of neonates produced that result from exposure to the test material.

5.3 Results of three-brood toxicity tests with C. dubia might be used to predict chronic or partial chronic effects on species in field situations as a result of exposure under comparable conditions.

5.4 Results of three-brood toxicity tests with C. dubia might be compared with the chronic sensitivities of different species and the chronic toxicities of different materials, and to study the effects of various environmental factors on results of such tests.

5.5 Results of three-brood toxicity tests with C. dubia might be useful for predicting the results of chronic tests on the same test material with the same species in another water or with another species in the same or a different water. Most such predictions are based on the results of acute toxicity tests, and so the usefulness of the results of a three-brood toxicity test with C. dubia might be greatly increased by also reporting the results of an acute toxicity test (see Guides E729 and E1192) conducted under the same conditions. In addition to conducting an acute test with unfed C. dubia, it might also be desirable to conduct an acute test in which the organisms are fed the same as in the three-brood test, to see if the presence of that concentration of that food affects the results of the acute test and the acute chronic ratio (see 10.4.1).

5.5.1 A 48 or 96-h EC50 or LC50 can sometimes be obtained from a three-brood toxicity test with a known test material, but often all the concentrations in the test will be below the EC50 or LC50. In addition, it is usually desirable to know the EC50 or LC50 before beginning the three-brood test, as a means to determine the concentrations for use in the chronic test (see 10.4.1). It should be noted that results from an acute test may not necessarily correspond to those of a chronic test, due to the addition of food to the chronic test.

5.6 Three-brood toxicity tests with C. du......

ASTM E1295-01(2013)相似标准